Ras Blockers Atherosclerosis Formation In The Early Arterial Blood Vessels Of Diabetic Rats,

Objective Diabetic macrovascular complication is one of the most common complication of the type-2 DM,which is also the main reason leading to be disabled or die.Artherosclerosis(AS)a independent risk factor,AS is one of the main reason caused the diabetic macrovascular complication and promoted it,however,the initial change of the AS is the damage of the vascular endothelial cell.We chose the rat as the subject for which has a similar way in the formation and patholog of the AS with human,we observed the diabetic rats and tested the activation and express of the Nuclear factor-kB (NF-kB)and Vascular cell adhesion molecule-1(VCAM-1),the variety of lympho-monocyte infiltrating in the endothelial in the early stage of the AS,explored the influence of the ACEI(Benazepril)and ARB(Irbesartan)—on the diabetic macrovascular complication of the rat in the early AS stage,and discussed the possible mechanism.Methods Forty male SD rats were randomly and equally divided into four groups: control group,diabetes mellitus group,Benazepril group and Irbesartan group.High lipid and high glucose were used for inducing DM in SD rats.The rats were raised for sixteen weeks.Total cholesterol(TC),Triglyceride(TG),Low density lipoprotein -cholesterol(LDL-C)and Lublood glucose(BG),Potassium glucose insulin(PGI)were measured.The plasma adiponectin level was measured using enzyme-linked immunosorbent assay(ELISA).The aortas were collected for histopathlogical and immunohistochemical studies.Immunohisto-chemistry was used to analyze the expression of NF-kB and VCAM-1 in the arterial vessel wall.Results The levels of TC,TG,LDL-C and BG of diabetes mellitus group,Benazepril group and Irbesartan group showed no difference,but were significantly higher than that of control group;The plasma adiponectin level was increased in Benazepril and Irbesartan group as compared with those of diabetes mellitus group (P＜0.01);NF-kB activation and VCAM-1 expression level and the monocytes infilitrating into the intima of the aortas in Benazepril and Irbesartan group was significantly higher than those in diabetes mellitus group(P＜0.01).The endothelial damage of the aortas in Benazepril and Irbesartan group was less severe than that in diabetes mellitus group.Conclusion Benazepril and Irbesartan can prevent early atherogenesis through alleviating the damage to the arterial wall by inhibiting the NF-kB,VCAM-1 expression and the monocytes infilitrating into the arterial wall.