Type 2 Diabetes Patients With Cerebral Infarction Of The Mthfr Gene And Enos Gene Polymorphism Loci

Objectives To investigate the association between C667T point mutation of N5,10 -methylene tetrahydrofolate reductase(MTHFR) gene,G894T point mutation of endodermis nitricoxide synthase(eNOS) gene and the patients with type 2 diabetes mellitus(T2DM) complicating cerebral infarction(CI).Methods Sequenom system is used to test Han national people' s MTHFR and eNOS genotype of 65 control groups,34 patients withT2DM and 42 patients withT2DM complicating CI in inner Mongolia.Results(1)general clinical data including mean age,sex,smoking etal get to matching among the control groups,patients with T2DM and patients with T2DM complicating CI groups well.(2)MTHFR gene C677Tsite' s frequencies of TT homogenesis and T allele(0.24,0.40) in patients with T2DM complicating CI have not significent difference with those in control(0.18,0.32) and those in T2DM(0.26,0.47),(P＞0.05).The frequencies of TT homogenesis and T allele in patients with T2DM have not significent difference with those in control,(P＞0.05).(3) eNOS gene G894T site's frequencies of TT homogenesis and T allele(0.14,0.26) in patients with T2DM complicating CI are significently higher than those in control(0.02,0. 11),there is significent difference(TTgenotype x~2 = 4.856,P = 0.028,OR:10.667, 95%CI:1.235-92.125;T allele x~2 = 8.673,P = 0.003,OR:2.940,95%CI:1.406-6. 148);the frequencies of TT homogenesis in patients with T2DM complicating CI have not significent difference with T2DM,the frequencies of T allele(0.26) there is significent difference compare with T2DM(0.12)(x~2 = 3.885,P = 0.049,OR:2.326,95%CI:0.991-5.462);the frequencies of TT homogenesis and T allele have not significent difference with those in control.(4)TTgenotype of eNOS gene G894T site and MTHFR gene C677Tsite have synergistic effect in the patients with T2DM complicating CI(x~2= 5.265,P = 0.04,OR: 12.000,95%CI:1.053136.794).conclusion MTHFR gene C677T and eNOS gene G894T site mutation increase chanciness of T2DM proceeding CI,they may be Hered predisposing genes.