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Co-mu Point Combination Studies, Rats With Gastric Ulcer Tissue Proteins By Sds-page Expression Profile Adjustment

Posted on:2009-08-18Degree:MasterType:Thesis
Country:ChinaCandidate:L ChenFull Text:PDF
GTID:2204360278454505Subject:Acupuncture and Massage
Abstract/Summary:PDF Full Text Request
Goal: The present paper in gathers solicits the theory and the clinical practice which the matching acupoint treatment stomach internal organs get sick has in the discovery foundation,and solicits the hole through the summary functioning of the stomach lower confluent acupoint blending and mainly treats the rule,and draws support from the SDS-PAGE technology thorough research and the revelation gathers solicits stomach internal organs disease and so on matching acupoint treatment gastric ulcer specificity effect rules and the function mechanism,blends for the acupoint with the acupoint coordination effect research provides the research mentality,and preliminary establishment acupuncture and moxibustion effect material base research new pattern and new technology.Method: 48 Wistar big mice divide into 6 groups stochastically: The blank group,the colligation group,the model group,"zu san li"group,"zhong wan"group,gather solicit the matching acupoint group.The gastric ulcer big mouse model uses the colligation to add the water immersion stress the method to make the model copy.Each group of big mice take the acupuncture point according to the grouping,gathers solicits the matching acupoint group to take"zu san li"Canada"zhong wan"the hole acupuncture;"zu san li"the acupuncture group takes"zu san li"the hole acupuncture;"zhong wan"the acupuncture group takes"zhong wan"the hole acupuncture;The model group first ties up every day,then plunges suddenly the big mouse sword below in the constant temperature waters;The colligation group similarly ties up every day;The blank group feeds normally,does not give processing.Various races big mouse stomach organization carries on the protein electrophoresis using the SDS-PAGE technology,the use image analysis software analysis not group difference expresses the protein.Results:1 visual observation colligation group,the model group have quantity not to wait for,a size ulcer stove not to appear,especially take model group as really.After the acupuncture treatment group treats the gastric mucosa extent of damage to be light,and gathers solicits the matching acupoint group effect to surpass the single hole acupuncture group.2 colligation groups,the model group,"zu san li"group,"zhong wan"group stomach protein electrophoretogram compares in the different position with the normal group or the model group has the varying degree color change.Gathers solicits the matching acupoint group stomach protein atlas and the normal group compares the not obvious difference.Conclusion:1 colligation water immersion stimulation can succeed the duplication irritability gastric ulcer big mouse model,and the effect surpasses the pure colligation stimulation.2 pure acupuncture"zu san li"holes or"zhong wan"hole can play certain role to the gastric ulcer model treatment,but is inferior to gather by far solicits the matching acupoint group curative effect to be obvious.
Keywords/Search Tags:Gastric ulcer, Gathers solicits the matching acupoint, zu san li, zhong wan, SDS-PAGE, Protein expression spectrum
PDF Full Text Request
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