Solid-Phase Extraction And Hplc For Analysis Of Trace Organic Pollutants In Environmental Water Samples

In recent years, the growth of the population and the scientific and technological level of the fast development, the increasing discharge of industrial, agricultural and sanitary waste lead to deterioration of water. The monitoring and control of the poisonous and harmful organic pollutants are the emphasis of environmental monitoring. Among 68 priority control pollutants of China, 58 pollutants are organic pollutants, including phthalic acid esters (PAEs) and polycyclic aromatic hydrocarbons (PAHs).Because of the complexity of matrix in water, and the low content of analytes, clean-up and enrichment procedures were required prior to analysis. Solid-phase extraction is a sample pretreatment method which is developed in recent years. Solid phase extraction (SPE), because of its advantages of high recovery, short extraction time, high enrichment factor, low consumption of organic solvents, and ease of automation and operation, was universally used for concentration of organic compounds in water samples. In this paper, SPE procedures coupled with high performance liquid chromatography (HPLC) were respectively developed for determination of 3 PAEs and 16 PAHs in environmental water.(1) The determination method of three kinds of PAEs in water, di(2-ethylhexyl)o -phthalate(DEHP), dimethyl o-phthalate (DMP), di-n-octyl o-phthalate (DOP), is proposed by SPE-HPLC. The five main influencing factors on recovery, that is, SPE column, elution solvent, elution volume, sampling speed and elutiion speed were studied. Under the optimal conditions, linear ranges, limits of detection (LOD) and relative standard deviations (RSD)of the method have been determined. This method was successfully applied to the analysis of tap water.(2) Bamboo charcoal, a new kind of material with special microporous, was used as solid phase extraction (SPE) adsorbent, coupled with monolithic column high performance liquid chromatography (HPLC), for high efficiency enrichment, fast determination of 16 polycyclic aromatic hydrocarbons (PAHs) in water. The key influence factors including elution solvents and volumes, flow rate of sample loading and sample loading volume were optimized to obtain high recoveries and extraction efficiency. The precisions and limits of detection of the method were determined. The optimized method was successfully applied to the determination of 16 PAHs in riverl water samples.