Separate Thermophilic Bacteria And Sludge High Temperature Composting

The water resources are very important for a country. But with the advance of China's industrialization process and the continuous development of China's social-economic. The environmental pollution of China has been very serious, and the status of water resources is more and more concerned, of which the sludge has particularly serious influence on water resources. How to make reasonable use of sludge and practical treatment of sludge is one of the major issues of our country and current world.This paper aims at separating the high temperature bacteria strains which can effectively produce protease and cellulose enzyme from the sample, analyzing and optimizing the high temperature bacteria strains enzyme production conditions through the experimental analysis and, gradually achieving the high temperature bacteria strains of enzyme production's maximum efficiency, testing their enzyme, and evaluating the potential of application.Secondly add the high temperature bacteria strains with efficient enzyme production capacity which separated from the sample to the sludge as biological additives, make the high-temperature composting after blending. Through a week or so, the sludge reaches the national ministry of agriculture composting of organic compost material standard, then make its reuse.Three strains were isolated from the sample, and they are all the coccus. They were separated for producing cellulase, designated as A1, A2and A3, with enzymatic activity of21.34U/ml,13.46U/ml,30.77U/ml,respectively. They also produce protease, with enzymatic activity of20.44U/ml,23.48U/ml,26.36U/ml, respectively.Respectively explore the optimized parameters for the three strains containing the types of carbon source, nitrogen source, the fermentation temperature and pH to A1, A2, A3three high temperature bacteria the influence of the enzyme activity. And draw A1, A2, A3three high temperature bacteria produce cellulose enzyme and protease maximum conditions.After optimizing the growth condition through single factor experiment, analyze the data of A1, A2, and A3. After the comprehensive consideration, the thermophilic bacterium in A2is the most efficient when producing the cellulase and protease. Then draw the conclusion by taking a medium components of orthogonal experiment after setting the temperature of the high temperature bacteria of A2as60℃, and medium with pH7.0.The composition of the medium of high temperature bacteria in A2includes glucose4g/L, sodium carboxy methyl cellulose11g/L, ammonium nitrate8g/L, specially designed2g/L, magnesium sulfate0.25g/L, potassium dihydrogen phosphate0.5g/L. The A2high-temperature bacteria grows most eugenic, and has the highest efficiency of producing cellulose enzyme and protease.According to the national machine fat rotten agent and health indicators of qualified conditions, expand the culture of A2high-temperature bacteria of liquid and of solid to conform the national standard. Set the temperature of solid compost above55℃for three days; the pH value is between7.2after composting; the bacterium number is2.4x1012; cellulose enzyme activity is260.11U/ml and protease activity is137.25U/m.Add the organic composting agents we made to the sludge as biological additives, then make high temperature composting with sludge and straws together. After high temperature composting for a week, the pH value everywhere after composting is between7.4; the bacterium number is2.6x1011; cellulose enzyme activity is336.67U/ml; protease activity is76.67U/ml.