Separation Of Flavonoids From Flaveria Bidentis (L.) Kuntze By Microporous Resins

In this paper, the performance and separation characteristics of ten differentmacroporous resins for the enrichment of flavonoids from Flaveria bidentis extractsare evaluated. The adsorption and desorption properties of these differentmacroporous resins are compared. Of them, D4020resin offers the best adsorptionand desorption capacity for total flavonoids as confirmed through experimentation. Inlight of this, dynamic adsorption and desorption experiments are carried out with thecolumn packed by D4020resin so as to optimize the separation process of totalflavonoids from Flaveria bidentis extracts.The optimum parameters for adsorption are as follows: sample solution concentration,2.72mg/mL, pH,4.5; processing volume,16bed volume (BV); and flow rate,2.12mg/mL; the optimum parameters for desorption are as follows: elution water,4BV;followed by ethanol–water (90:10, v/v ratio),5BV elution. After separation withD4020resin, the content of flavonoids in the product increases from4.3%to30.1%,with a recovery yield of90%. D4020resin can also basically separate and enrichisorhamnetin3-sulphate and astragalin. After the treatment with gradient elution onD4020resin, the content of isorhamnetin3-sulphate in the product increases from0.49%to8.70%, and the recovery yield is74.1%; the content of astragalin increasesfrom1.16%to30.8%, with a recovery yield of92.2%.This study also assesses the adsorption and desorption properties of flavonoids ondifferent macroporous resins in order to achieve an efficient method for preparativeseparation of total flavonoids, isorhamnetin3-sulphate and astragalin from crudeextracts of Flaveria bidentis. Moreover, a generation revision of the biological andecological characteristics of this plant, coupled with its separation and analyticalmethods, is contained herein.