Experimental Study Of Sperm DNA Oxidative Damage Of Idiopathic Asthenozoospermia Patients

Objective:Discuss seminal plasma oxidative stress of idiopathic asthenozoospermia patients and sperm DNA oxidative damage.Methods:ALL objects of study are from the outpatient clinic of the reproductive center of Xiangya Hospital Central-south University from December 2010 to March 2011. We collected the fresh semens of 28 idiopathic asthenozoospermia patients as study group, and 24 normal semen analysis and proven fertility men as control group. ROS level in seminal plasma is assessed with luminol chemiluminescence. Density of sperm DNA oxidation product 8-OHdG is assessed with enzyme linked immunosorbent assay (ELISA).Result:1. ROS level in the study groups are higher than the control group (P <0.05). There is negative correlation between percentage of progressive motility spermatozoa and ROS level in seminal plasma(P<0.01).2. Density of sperm 8-OHdG in the study group is higher than the control group(P<0.05). There is negative correlation between percentage of progressive motility spermatozoa and density of sperm 8-OHdG(P< 0.01).3. There is positive correlation between ROS level in seminal plasma and density of sperm 8-OHdG(P<0.01). Conclusion:1. There is sperm DNA oxidative damage in idiopathic asthenozoospermia patients, and it may be correlation with oxidative stress.2. Excessive reactive oxygen species generation may be a cause of sperm vitality decreased of idiopathic asthenozoospermia patients.