Study On Abstracted And Depurated And Lipid-Lowering Effects Of Earthworm Fibrinolytic Enzyme

To explore the lipid-lowering effects of Earthworm fibrinolytic enzyme on hyperlipidemia of mice. Earthworm fibrinolytic enzyme is purified in this study, and hyperlipidemia was detected in mice on serum and liver tissue and liver biochemical markers of cell adhesion I of molecule immunohistochemistry. earthworm fibrinolytic enzyme induced by high fat diet fed mice hyperlipidemia prevention, treatment effect.1. Fibrinolytic enzyme purifiedAmmonium sulfate precipitation of crude extract of earthworm fibrinolytic enzyme extracted using SephadexG-75 gel filtration chromatography and DEAE-sepharose Fast Flow anion-exchange chromatography of earthworm fibrinolytic enzyme, protein activity using BAEE method, collected 2 An activity peak proteins, using SDS-Page detection of protein molecular weight, respectively 33KD; the DEAE-sepharose Fast Flow chromatography, the product was diluted with saline was prepared by plasmin preparations.2. Animal experiments:Fibrinolytic agents used for prevention of experimental mice, the results show that:earthworm fibrinolytic enzyme on the formation of lipid disorders have a good preventive effect; mice significantly decreased TC, TG, LDL, AST, MDA and other indicators to improve the small Rat HDL, LPL indicators and SOD activity; Immunohistochemistry results showed that earthworm fibrinolytic enzyme can effectively reduce the liver cell adhesion molecule expression of I; preventive effect of the agent in the appropriate dose of enzyme.Fibrinolytic agents used for treatment of hyperlipidemic mice, the results show that:earthworm fibrinolytic treatment of mice with high viremia have a good effect; mice significantly decreased TC, TG, ALT and other indicators, increased LPL, HL, SOD activity; Immunohistochemistry results showed that earthworm fibrinolytic enzyme can significantly reduce the liver cell adhesion molecule expression of I; treatment dose to the dose of enzyme agent for the best.3.Conclusion:earthworm fibrinolytic enzyme for the good of hyperlipidemia and significant preventive effect of treatment. The mechanism may be by reducing lipid absorption. Accelerate the excretion of hepatic cholesterol, regulate cholesterol metabolism and lipoprotein metabolism to promote the transformation of triglyceride metabolism and accelerate the transformation of liver cells and reduce repair and stable cell adhesion molecule expression ofâ… several aspects.