Regulating Action Of Drugs For Regulating Qi Flow Fructus Aurantii Immaturus On Cerebral Infarction At Acute Period Complicated With Gastrointestinal Dysfuction

Objectives:To observe the presentence of gastrointestinal dysfunction,to discuss the pathogenesis of cerebral infarction at acute period complicated with gastrointestinal dysfunction and investigate the possible effects of drugs for regulating Qi flow Fructus Aurantii Immaturus(FAI) in rats, in order to provide experimental basis for its clinical use.Methods:Forty modles of middle cerebral artery occlusion(MCAo)were made. They were divided into Cerebral infarction(CI) group,FAI group,Cimetidine group randomly, which were given Sodiun Chloride,FAI,Cimetidine.Another 24 rats which were made into sham operation group,24 normal rats as normal control group,were given Sodiun Chloride.According to the time when the rats were killed,CI group,sham operation group,normal control group will be divided into 24h,48h,4d 3 subgroups separately.FAI group and Cimetidine group were executed after they were introduced to MCAo for 4d.There was a total of 11 groups and 8 rats in each group.In the fasting state, inferior vena venous blood samples were collected to detect the Plasma gastrin (GAS),motilin (MTL) and vasoactive intestinal peptide (VIP) concentrations by radioimmunoassay (RIA).After the rats were decapitated,the fresh gastric mucosa gastric were collected immediately,fixed in 10% neutral formalin,2.5% glutaraldehyde and observed with the light and the electron microscopy.Results:1.Plasma MTL,serum GAS and plasma VIP in all CI rats,were higher than rats of normal group (P<0.01).In CI groups,with the time going on,serum GAS decreased;MTL and VIP in plasma at CI 48h group continued to rise and significantly decreased at CI 4d group,which was less than CI 24h group (P<0.01).2.Serum GAS of FAI group and cimetidine group were significantly lower than CI 4d group (p<0.01),but still higher than normal group (p<0.01),there were no statistical difference between the two groups (p>0.05).Plasma MTL and VIP of FAI group were lower than CI 4d group significantly (p<0.01),cimetidine group concentration also decreased,but there was no significant (p>0.05).Plasma MTL and VIP of FAI group and cimetidine group concentration were still higher than normal group (p<0.01).Compared with cimetidine group,FAI group can decreased in plasma MTL (p<0.01) and VIP (p<0.05) concentration significantly.3.In CI 24h group and CI 48h group,there were visible damages at gastric mucosa. Under the light microscope,ulcers and necrosis were found,there were a large number of inflammatory cell infiltration, marked congestion and edema at the mucosa and submucosa. Gastric mucosal tissue of CI 4d group injured improved,there were no ulcer,necrosis,no significant change with the unaided eyes.Under the light electron microscope,gastric mucosal tissue gastric of CI 4d group rats were seen serious shedding epithelial cells,exposed basal layer,exposed collagen fibers in part and gastric pits severely damaged.4.Gastric mucosal tissue of FAI group and cimetidine group rats did not change significantly with the unaided eyes.Under the light microscope,the infiltration of the inflammatory cells,telangiectasia,congestion,edema at the mucosa and submucosa were improved significantly in FAI group rats,there were no erosion,ulceration and necrosis at gastric mucosal tissue.Gastric mucosal tissue of cimetidine group rats still were seen infiltration of inflammatory cells significantly,marked congestion and edema.Under the electron microscope,at mucous of FAI group rats,more complete gastric epithelial cells can be seen,there is no significant deformationd at mucous and gastric pits is in order.Gastric mucosal tissue of cimetidine group rats were seen flat, shedding epithelial cells,exposed the basal layer, the deformation of the epithelial cells covered with microvilli around.Conclusions:1.There were pathological changes in the acute stage of cerebral infarction in rats gastric mucosal morphology.2.The change of gastrointestinal hormone may be one of the main pathogenesis of gastrointestinal dysfunction in acute cerebral infarction.3.Early use FAI on gastrointestinal dysfunction in the acute stage of cerebral infarction has a protective effect.