Dynamic Analysis And Research On The Quality Of Fructus Aurantii And Fructus Aurantii Immaturus Medicinal Materials

Fructus Aurantii,as an authentic medicinal material in Jiangxi Province,has stable quality,good quality and good curative effect compared with Fructus Aurantii produced in other producing areas.Fructus Aurantii and Fructus Aurantii immaturus have the same basic source,but they show similar but different composition characteristics and clinical effects in different growth stages,so they are listed as two different medicinal herbs in Chinese pharmacopoeia.The harvesting indexes of medicinal materials were different among different varieties,countries and climatic conditions.In recent years,with the increase in the demand for Fructus Aurantii and the shortage of wild resources,the disorderly development of Fructus Aurantii,the lack of variety innovation,the quality and safety level is not high,affecting the green and healthy development of TRADITIONAL Chinese medicine.Therefore,this article use the fingerprint and composition analysis to select the region(ground),and explore this region(ground),the dynamic changes of the different periods of acid-insoluble ash composition,to determine the best harvest time of Fructus Aurantii immaturus,and to explore the efficacy of material base.1.Study on origin of Fructus Aurantii(1)Comparative study on Fructus AurantiiThe quality of Fructus Aurantii was evaluated by HPLC fingerprint and multicomponent content determination,combined with chemical pattern recognition technology.The fingerprint of Fructus Aurantii was established,and 17 common peaks were calibrated,and the similarity of 24 batches of Fructus Aurantii ranged from 0.965 to 0.998.Eight signature compounds with VIP value >1 were screened out by partial least squares discriminant analysis.The content of identified chromatographic peaks was determined.The best producing area is Zhangshu Wucheng in Jiangxi Province.(2)Study on non-volatile components of Fructus AurantiiThe samples were ultrasonically extracted with 70% methanol,and positive and negative ion scanning was carried out by UPLC-Q-TOF-MS.A total of 30 compounds were identified,most of which were flavonoids.(3)Study on volatile components of Fructus AurantiiThe volatile oil of Fructus Aurantii from Jiangxi,Chongqing,Hunan and Zhejiang provinces was detected by GC-MS,and 24 chemical components were identified.The contents of limonene,γ-terpinene and linalool are high.(4)Study on network pharmacology mechanism of volatile oil from Fructus Aurantii in the treatment of functional dyspepsiaUsing network pharmacology of hovenia dulcis naphtha active ingredient in the treatment of functional dyspepsia(FD),database,such as Genecard hovenia dulcis naphtha composition > 1%,for active ingredients,to predict potential related targets for the treatment of functional dyspepsia,build a PPI,filter core gene,construct hovenia dulcis naphtha pathway for the treatment of FD diagram,The results showed that Fructus Aurantii volatile oil is multi-target and multi-pathway to treat FD.2.Best harvest time of Fructus Aurantii immaturus(1)Study on fingerprint of Fructus Aurantii immaturusUsing HPLC to establish four origin 24 batches of samples of Fructus Aurantii immaturus fingerprint,calibration of 17 common peak,identify the 7 characteristic peak,24 batches of herbs similarity were greater than 0.930,can provide scientific basis for the medicinal materials quality standard Fructus Aurantii immaturus.(2)Analysis of non-volatile components of Fructus Aurantii immaturusThe samples were ultrasonically extracted with 70% methanol and scanned with positive and negative ions by UPLC-Q-TOF-MS.A total of 22 compounds were identified,with the largest number of flavonoids and a small number of alkaloids,cardamine and limonin.(3)Study on volatile components of Fructus Aurantii immaturusThe volatile oil from Jiangxi,Chongqing,Hunan and Zhejiang Poncirus aurantii was detected by GC-MS and 27 chemical components were identified.The contents of D-limonene,γ-terpinene,linalool,4-terpenol and β-pinene in the volatile oil of Fructus Aurantii immaturus were high.(4)Network pharmacology mechanism of volatile oil of Fructus Aurantii immaturus in the treatment of functional dyspepsiaUsing network pharmacology of acid-insoluble ash naphtha active ingredient in the treatment of functional dyspepsia(FD),database,such as Genecard acid-insoluble ash naphtha composition > 1% for active ingredients,to predict potential related targets for the treatment of functional dyspepsia,build a PPI,filter core gene,build the naphtha pathway for the treatment of FD Fructus Aurantii immaturus figure,The results showed that the volatile oil of Fructus Aurantii immaturus was multi-target and multi-pathway in the treatment of FD.(5)The best harvest time of Fructus Aurantii immaturus Different harvest time of acid-insoluble ash was established based on HPLC fingerprint,determines the chromatographic peak of seven of the fingerprint characteristic,recycle chemical pattern were analyzed,and the screening of VIP chromatographic peak value >1,the identification of chromatographic peak and content determination.According to the regulation of Chinese Pharmacopoeia of "Syneferine ≥0.3% and 0.5-2.5cm in diameter",the harvesting period of Fructus Aurantii immaturus was determined from May 29 to June 26 in Fuzhou,Jiangxi province.According to the Provisions of The Chinese Pharmacopoeia,"naringin ≥4.0%,neohesperidin ≥3.0% and diameter 3-5cm",combined with the content determination results and fruit diameter size,we can determine the Fructus Aurantii in Fuzhou,Jiangxi province from July 3 to July 24.