The Effect Of Shuyu Capsule On The Location And Expression Of 5-HT3B Recepter In Different Brain Regions Of Depression Emotion Model Rats

Objective:Our aim is to detect the relationship between protein location & expression levels of 5-HT_3BR in correlated region offrontal cortex , parietal cortex ,hippocampus & hypothalamus of depression emotion model rats, which gives a preliminary discussion on the pathogenesis of depression emotion,as well as studies the effects of Shuyu capsule.Methods:After being chosen,depression emotion model rats were prepared by using chronicity mild stimulus(CMS)method.And then the rats were evaluated by sucrose preference test,open field test and macro-behavior observation.We used fluoxetine as masculine medicine, detected the 5-HT_3BR protein location in parietal cortex, frontal cortex, hippocampus and hypothalamus of rats in the groups including the normal group, depression emotion model group,and the two treatment groups by using immunofluorescence(IF), and detected the mean fluorescence intensity (MFI) in CA1,CA3 of hippocampus and in ventromedial hypothalamic area(VMH)of rats.Results: Compared with the normal group,the weight of rats in depression emotion model group lessened significantly(P<0.01), the crossing score,rearing score and total score of open field test all decreased obviously(P<0.01) and the sucrose intake of preference test decreased obviously(P<0.01).Meanwhile, the rats showed listlessness,dull in eyes and insensitive to surrounding stimulation.On the other hand,compared with the depression emotion model group,the weight of rats in Shuyu capsule and fluoxetine treatment groups increased significantly(P<0.05), the open field scores all increased remarkably(P<0.01),and the sucrose intake of preference test increased obviously(P<0.01),and there was no statistical differences in the two treatment groups(P>0.05).IF demonstrated that there was no noticeable differences in location of 5-HT_3BR infrontal cortex, parietal cortex, hippocampus and hypothalamus of rats in four groups.Compared with the normal group,the numerical value of MFI of 5-HT_3BR in depression emotion model group increased markedly (P<0.05 or P<0.01)in CA1,CA3 of hippocampus and in VMH, there maybe a elevated possibility of 5-HT_3BR in frontal cortex and parietal cortex. Compared with the depression emotion model group, the numerical value of MFI of 5-HT_3BR in Shuyu capsule and fluoxetine treatment groups decreased markedly (P<0.05)in CA1 of hippocampus and in VMH, there was no statistical differences in the two treatment groups(P>0.05), and there maybe a reducing possibility of 5-HT_3BR in frontal cortex and parietal cortex.Conclusion: The model rats with depression emotion can be prepared successfully by using chronicity mild stimulus(CMS),and they can be evaluated effectively by sucrose preference test,open field test and macro-behavior observation.The high expressions of the5-HT_3BR protein in frontal cortex, parietal cortex, CA1,CA3 of hippocampus and in VMH may have close relationship with depression emotion,and probably is one of the centre pathogenesis of depression emotion.The Shuyu capsule can correct the high expression of 5-HT_3BR protein of model rats in above brain regions, which may be one of the mechanism to treat depression emotion.