The Mechanism Of Hypothalamus, MVZ And Vagus Nerve In Multiple Organ Dysfunction Syndrome Model Induced By Cerebral Hemorrhage Of Rats

ObjectiveTo establish a model of cerebrogenic multiple organ dysfunction syndrome of the rat induced by cerebral hemorrhage. To observe pathological changes of lung, liver, small intestine and renal in rats, The expression of FOS in the hypothalamus of the rats were examined with immunohisto chemistry. The expression of CHAT in the medullary visceral zone of the rats were examined with immunohisto chemistry.To investigate the role of the hypothalamus, MVZ and vagus in cerebral hemorrhage (CH) of multiple organ dysfunction syndrome (MODS) rat model. To revealinghe the possible regulatory mechanism of the Hypothalamus—MVZ—vagus pathway in CMODS.Methods1.80 wistar rats were randomly divided into sham-operative group (n=10), subdiaphragmatic vagotomy group (SDV) (n=10), CH group (n=20), CH+SDV group (n=20) and CH+vagus stimulation group(CH+SIV) (n=20).2. CH rat model was made by injection mixture of collagenase 0.8 U and 3.5 IU of heparin sodium in caudate nucleus, SDV rats were anesthetized and underwent laparotomy surgery, to expose two vagus nerve at lesser gastric curvature, tigate the vagus nerve with silk thread and cut off it at a distance of about 5 mm from each branch. To expose the vagus of SIV rats at bilateral neck.The silver electrode was made like a ring, brush the outer ring handle with insulating paint, the inside of the ring is electrode surface, withhold the vagus nerve by the ring, purfuse paraffin oil in order to make the surrounding tissue insulation, sutured incision at last. 3. Record the temperature, heart rate, respiratory rate,WBC and other symptoms and signs of changes, detect the liver, renal function and myocardial enzyme changes,observe the pathological changes of lung, liver, kidney and small intestine by microscope, to determine SIRS, MODS incidence according to diagnostic criteria.4. The expression of FOS in the hypothalamus of the rats were examined with immunohisto chemistry.5. The expression of CHAT in the medullary visceral zone of the rats were examined with immunohisto chemistry.Results1. Incidence of SIRS in CH group, CH+SDV group and the CH+SIV group is 100%, the incidences of MODS in CH group is 70%, the incidences of MODS in CH +SDV Groups is 75%, the incidences of MODS in CH+SIV group is 65%.2. Abnormality of hepatic and renal function, myocardium enzyme and the pathological change of liver, kidney, lung,small intestine in CH+SDV group, CH+SIV group and those of CH group outweigh than sham-operative group and SDV group, CH+SDV group outweighs than CH group. CH+SIV group were much lighter than those of CH group.3. The positive expression of FOS in hypothalamus of CH+SDV group were much higher than those of sham-operative group (9.3±2.6 and 2.5±0.4, t=8.17, P<0.01) and SDV group (9.3±2.6 and 2.9±0.5, t=7.64, P<0.01), but much fewer than those of CH group (9.3±2.6 and 48.6±18.1, t=-6.80, P<0.01),CH+SIV group were more than those of CH group (74.25±6.29 and 48.6±18.1, t=4.23, P<0.01).4. The positive expression of CHAT in MVZ of CH+SDV group were much higher than those of sham-operative group (138.49+11.76 and 108.15±9.54, t=5.39, P<0.01) and SDV group (138.49±11.76 and 110.71±9.88, t=4.83, P<0.01), but much fewer than those of CH group (138.49±11.76 and 164.42±12.44, t=-3.53, P<0.01), CH+SIV group were more than those of CH group (189.38±14.97 and 164.42±12.44, t=3.14,P<0.05). Conclusion The hypothalamus—MVZ—vagus pathway may be distinctive in CMODS. Vagus nerve may be a Bi-directional Channel of the rats following cerebral hemorrhage and multiple organ dysfunction syndrome.