Short-term Curative Efficacy Of Cytokine Induced Killer Cells On Patients With Gynecological Malignant Carcinoma

Objective:To observe the short-term curative efficacy and safty of transfusing autogeneic cytokine induced killer cells(CIK) cultured in vitro on patients with gynecological malignant carcinoma.Methods:(一) In vitroPeripheral blood mononuclear cells(PBMC) isolated from 8 healthy people were induced in vitro to obtain CIK cells. Trypan blue staining was used to observe the proliferation of CIK cells since the 1st training day to the 20th day. The cytotoxic activities of CIK cells against SKOV3, Hela, and Ishikawa were measured respectively using lactate dehydrogenase(LDH) assay on the 14th training day of CIK cells.(二) Clinical trailPeripheral blood isolated from 8 patients with gynecological malignant carcinoma were induced in vitro to obtain CIK cells which were then transfused to the patients three times. Trypan blue staining was used to observe the proliferation of CIK cells since the 1st training day to the 14th day. Peripheral blood were obtained to detect the imunological function, including T lymphocyte subset countin, NK subpopulation, NKT subpopulation and T regulation lymphocytes subpopulation which were measured by flow cytometry before and two months after the transfusion. The level of CA-125 in serum was detected before and two months after the transfusion. Karnofsky performance score(KPS) and the change of clinical symptoms were detected before and two months after the transfusion. Adverse reaction was observed closely during the whole process.6 patients with gynecological malignant carcinoma were used as control group. Peripheral blood were obtained to detect the imunological function, including T lymphocyte subset countin, NK subpopulation, NKT subpopulation and T regulation lymphocytes subpopulation which were measured by flow cytometry before and 7～10 days after the 1 st term chemotherapy.Results:(一) In vitroCIK cells were induced sucessfully in vitro by y-IFN, CD3Ab and rhIL-2. As the increasing of the effect-target ratio between 2.5:1 and 40:1, the cytotoxic activities of CIK cells against tumor cells were increased.(二) Clinical trailTwo months after the transfusion, both the ratio of CD4+/CD8+ and the percentage of CD3-CD16+CD56+in peripheral blood were increased (P<0.05) while the percentage of CD4+CD25+T cells were decreased (P<0.05) in experimental group. The percentage of CD3+T cells, the ratio of CD4+/CD8+ and the percentage of CD3-CD16+CD56+in peripheral blood were all decreased (P<0.05) before and 7 10 days after the 1st term chemotherapy in control group. Two months after the transfusion, the levels of CA-125 in serum were decreased in 5 cases. Two months after the transfusion, the KPS was increased in one case and clinical symptoms were improved in 5 cases. No adverse reaction was observed.Conclusions:(1) Transfusing autogeneic CIK cells do has some short-term curative efficacy against gynecological malignant carcinoma.(2) Transfusing autogeneic CIK cells is safe.