PIAS3 Expression In Development Of Gastric Carcinoma

Objective:To study the expression of PIAS3 (protein inhibitor of activated signal transducers and activators of transcription 3) in evolutionary process of gastric cancer.Methods:Samples were taken from the endoscopic biopsy specimens of 125 patients. Gastric mucosal lesions were diagnosed in HE staining, and chronic atrophic gastritis (CAG) with intestinal metaplasia (IM) were distinguished in AB-PAS and HID-AB staining. The expressions of PIAS3 gene in different types of gastric mucosal lesions were detected by immunocytochemistry and in situ hybridization. The results were analyzed using IPP 6.0 image analysis system, from which the average optical density was obtained of positive cells.Results:There were 25 patients with chronic superficial gastritis (CSG),87 CAG (30 with complete intestinal IM,27 with incomplete intestinal IM,21 with complete colonic IM,9 with incomplete colonic IM),8 dysplasia (DYS) and 5 gastric cancer (GC). The average optical density of PIAS3 protein in NAG, CⅡM,ⅢM, CCIM, ICIM, DYS and GC were 21.19±0.49,19.55±0.61,19.76±0.64,17.37±0.77, 13.51±0.54,8.03±0.38 and 5.73±0.42, and those of mRNA were 9.42±0.24, 9.21±0.29,9.00±0.35,7.25±0.41,5.22±0.26,3.88±0.18,2.71±0.13. In the expressions of PIAS3 mRNA and protein, a difference was not found between patients with CSG and with CAG with complete or incomplete intestinal IM; however, a significant difference was statistically found among patients with CSG (or intestinal IM), complete colonic IM, incomplete colonic IM, DYS and GC, expression levels of which steped down one by one. Conclusions:There are differences in the PIAS3 expression from different stages of gastric precancerous conditions/lesions to GC, which may reveal a close relationship between expression reduction or loss of PIAS3 and gastric tumorigenesis.