Effects And Mechanisms Of Prevention And Cure Of Rat Liver Fibrosis With LDP

Hepatocirrhosis is a hackneyed kind of chronic, progressing and penetrating liver desease. Casued by many kinds of pathogeny recrudescing long term or repeating, it's a disease which is hard to cure and harm human health gravely. In China, hepatocirrhosis is mostly caused by hepatitis virus, but the incidence of alcohol caused hepatocirrhosis is increasing every year recently. Liver fibrosis means hyperplasia and abnormal aggradations of ECM in liver tissue, which cause the pathology change of liver structure and function abnormity. In structure, it is presented as capillary vessel of liver antrum, and fibrosis of liver foliole and portal area. In function, it is expressed as hypohepatia, high pressure of portal vein and so on. The mechanisms of liver fibrosis are trauma factors such as hepatitis virus, ethanol, drugs, schistosome, metabolize and inherits, fill up of bile, immunosuppressive, hurt liver long last and chronic, cause the activation of hepatic stellate cells in liver antrum, and unbalance of ECM, compose greater than decompose decompose, finally cause the aggradation of ECM and destruct of tissue structure. Liver fibrosis can be found mostly in chronic liver disease cause by different kinds of pathogeny, hepatocirrhosis can be formed with further development, which influence the health and lives of sufferers strictly.The consideration of hepatocirrhosis in TCM is firstly Humid Heat and pestilence, or stasis of liver blood caused by dysfunction of Qi, and then the weak of ZhengQI, so the therapy is mostly proceed with Fuzheng Huayu. Composed by Lingzhi, Dansen, Tianjihuang, Kusen, Danggui, Chaihu, Wuweizi, Biejia, Shanyao, CH compound Ling-dan Powder has the ability of Fuzheng, Rougan and Lishui. The former research has indicated that AQP-1 ties up with the form of ascites. The three taches of clinic therapy of hepatocirrhosis are paid great attention in this paper: liver fibrosis, icterus and ascites, disorders of functions of stomach and intestines. At the points of preparations of TCM, clinic curative effect and mechanisms of pharmacology, LDP is taken as observing medicine, compound Biejiaruanganpian is taken as comparing medicine, and liver damaged rats are prepared by admixture of 60% CCl4 and olive oil. Methods such as immunohistochemistry, PT-PCR are adopted to test the presentation of AQP-1 albumen and mRNA in epithelium of hepatic duct and endothelial cell membrane of peritoneum capillary vessel. Mechanism of the effect of LDP is discussed finally. The aims are: 1. Observing the clinical curative effect of CH on prevention and cure hepatocirrhosis(resist HF), and offering data for developing this medicine; 2. Uncovering the new mechanism of TCM"Lishui/Lishi". Parts of the essence of CH"Lishui/Lishi"are explained by results of this research, news methods to prevention and cure hepatocirrhosis by TCM are presented, which is active for superiority of assimilation of TCM internal medicine. The purports of this paper are: 1. testing the influences of Lingdansan on liver functions and indexes of liver fibrosis of CCl4 induced hepatocirrhosis rats, observing the general curative effect of LDP; 2. Observing the influences of LDP on presentation of AQP-1 on peritoneum, in order to investigate molecular biology mechanism of Lishui/Lishi effect.Experimentâ… Effects of LDP on pathological of liver tissue, liver function indexes and liver fibrosisObjective: To discuss the effects of LDP on pathological of liver tissue, liver function indexes and liver fibrosis. Methods: 60 rats were divided into normal group, hepatocirrhosis group, hepatocirrhosis model group, compound Biejiaruanganpian treated group, LDP treated group with large, middle and low dosage, 10 rats each. Cytotoxicity hepatocirrhosis rat model was induced by hypodermic CCl4. The treated group started to be gavaged with CH at the fourth week, and lasted for four weeks. Pathological changes of liver fibrosis were observed by HE staining. The activity of AST, ALT, Alb of serum were detected by Kit. Four indexes of hepatocirrhosis, which included hyaluronic(HA), laminin(LN), collagenâ…£(C-â…£) and procollagenâ…¢(PCâ…¢), were detected by radio immunoassay method. Results: Compared with normal group, the liver functions of model group were abnormal, pathological changes of liver fibrosis were obvious. Liver functions and pathological changes of liver fibrosis were amended remarkably by Lingdansan in the model group. Four indexes of liver fibrosis increased significantly.Experimentâ…¡Effects of LDP's adjusting on expression of AQP-1 and mRNA of peritoneum and liver tissue Objective: To discuss the effects of LDP's adjusting on expression of AQP-1 and mRNA of peritoneum and liver tissue. Methods: Tailor tissue paraffin section, then gather and analyze the images through immunohistochemistry. Distill total RNA to test A value of RNA sample, calculate concentration and then retroviral it. Finally, gather and analyze the images after DNA amplification and PCT circulation. Results: AQP-1 is mainly expressed on endothelial cell membrane of peritoneum capillary vessel. The expression of AQP-1 of model group is clearly lower than the normal group, but expression of treated group is elevated to the normal group approximately. Expression of mRNA of model group is clearly lower than others, and the expression of treated group grows up to normal.Conclusions:LDP can mend the changes of liver functions and fibrosis of CCl4 induced liver fibrosis rats significantly, and raises the expression of AQP-1 and mRNA, increases the reabsorbtion of water by peritoneum, which is maybe the mechanism of CMM Lishui/Lishi.