The Expression And Clinical Significance Of Motility-related Protein (MRP-1/CD9) And MMP-2 In Squamous Cell Carcinoma Of The Tongue

Background:Cancer is a common disease that serious threat to people's health, According to statistics, oral and maxillofacial malignant tumor accounting for 8.2% of the body malignant tumor.squamous cell carcinoma of the tongue is one of the mostly common primary site in oral cavity and is characterized by a high degree of local aggression and metastasis to cervical lymph nodes. Therefore, to investigate the associated lymph node metastasis protein of the squamous cell carcinoma of the tongue, has important basic and clinical significance.Objective:To investigate the expression of motility-related protein(MRP-1/CD9) and matrix metalloproteinase-2(MMP-2) in squamous cell carcinoma of the tongue, to analyse theie correlation with clinical stage, histological types and grades, lymph nodes metastasis of the squamous cell carcinoma of the tongue,and to explore the mechanism of incidence,invasion and metastasis.Methods:Using Envision immunohistochemical method,53 squamous cell carcinoma of the tongue specimens were tested for expressions of MRP-1/CD9 and MMP-2,Correlations with clinicopathological parameters and prognostic significance were tested statistically. All data were statistically analyzed by SPSS 17.0,usingχ2 test. The difference was considered significant as P< 0.05; The relationship between protein levels expression was evaluated using the Mann-Whitney test.Results:1,The positive expression rates of MRP-1/CD9 and MMP-2 in squamous cell carcinoma of the tongue were 60.4%(32/53) and 62.3%(33/53).2,MRP-1/CD9 protein was associated with lymph node stage of the tumor(P< 0.05). The levels of MRP-1/CD9 expression were found no significant differences in sex, different clinical stages, different histological grades and different histological types(P> 0.05). The positive expression rate of MRP-1/CD9 with no lymph node metastasis in squamous cell carcinoma of the tongue was 78.26%(18/23),which was significantly higterlower than those lymph node metastasis(50%,14/28). With positive expression of MRP-1/CD9, the lymph nodes metastasis rate was low, while with downregulated expression of MRP-1/CD9, the lymph nodes metastasis rate was high.3,MMP-2 protein was associated with lymph node stage and pathological stage(P<0.05). The levels of MMP-2 expression were found no significant differences in sex and different clinical stages(P>0.05). The positive expression rate of MMP-2 with no lymph node metastasis in squamous cell carcinoma of the tongue was 47.83%(11/23),which was significantly lower than those lymph node metastasis (78.57%,22/28).Patients with positive MMP-2 expression had higher lymph nodes metastasis rate, while with downregulated expression of MMP-2, the lymph nodes metastasis rate was lower.4,The higher the MRP-1/CD9 expression, the higher the survival rate. MRP-1/CD9 protein expression was significantly correlated with 5-year survival rate(P< 0.05).5,the higher the MMP-2 expression, the lower the survival rate. MMP-2 protein expression was significantly correlated with 5-year survival rate(P<0.05).6,The expression of MRP-1/CD9 was closely correlated with that of MMP-2(P <0.05).Conclusion:1,MRP-1/CD9 protein was associated with lymph node stage of the tumor. The levels of MRP-1/CD9 expression were found no significant differences in sex, clinical stages, histological grades and histological types.2,MMP-2 protein was associated with lymph node stage and pathological stage. The levels of MMP-2 expression were found no significant differences in sex and clinical stages.3,The expression of MRP-1/CD9 was significantly negatively correlative to the expression of MMP-2.4,The detection of MRP-1/CD9 and MMP-2 expression can provide reference for the prognosis of the squamous cell carcinoma of the tongue.