1.The Epidemiology Study Of Metabolic Syndrome 2.The Change Of Serum MMP-2 Level In Patients With Metabolic Syndrome And Discussion Of Its Mechanism

ObjectiveTo survey the prevalence and risk factors of MS among the dwellers in Longfeng and Sushe communities of Haizhu district in Guangzhou.MethodsA cluster-random-sampling survey was performed from August to October in 2009 in the two communities by using the same protocol and questionnaire. Total 2003 dwellers aged over 35 years were enrolled. Data was calculated by SPSS 13.0 package via methods of binary logistic regression, chi-square-est and t-test. The prevalence were age-standardized according to the fifth population census.Results1. There were 2003 samples with mean age of 58.4±11.6, 788 men and 1215 women with mean age of 59.9±11.6 and 57.4±11.5 respectively; There was significant difference in age (63.0±9.9 vs. 56.3±11.5, P<0.001) beteween MS and non-MS.2. The crude prevalence of metabolic syndrome were 15.8%, 28.5% and 30.3%, according to the criterion of CDS, IDF, ATPⅢrespectively. The age-standardized prevalence were 11.2%, 22.2% and 23.7%. The prevalences of MS were increased with age. Compared with male, female had higher morbility of MS;3. The age-standardized morbility of abdominal obesity, obesity, diabetes, impaired fasting glucose regulation, hypertension, high blood TG and low blood HDL-C were 37.5%, 27.7%, 6.8%, 6.0%, 26.1%, 36.6% and 10.5% respectively. The prevalence of MS and its risk factors increased with age;4. Hypertension, disorder of glucose regulation, obesity, dislipidemia and high blood uric acid were analyzed as five metabolic disorders. About 2/3 individuals of two communities had one or more than one of the metabolic disorders, 2/5 individuals had two or more than two metabolic disorders and 1/5 individuals had three or more than three metabolic disorders;5. The combination of obesity, dislipidemia and hypertension was the most common combination of MS individual components. It accounted for 41.5%. The combination of obesity, disorder of glucose regulation, dislipidemia and hypertension was the second(21.5%). The third was the combination of dislipidemia, hypertension and disorder of glucose regulation (16.5%). Both the combination of obesity, disorder of glucose regulation and hypertension and the combination of obesity, disorder of glucose regulation and dislipidemia accounted for 10.4%;6. Visceral obesity was the most important risk factors of MS. UA, TC, history of hypertension and WBC were also risk factors.Conclusions1. The prevalence of MS with CDS standard< that with IDF standard<that with ATPⅢstandard;2. Compared with male, female had higher morbility of MS;3. The morbility of MS and its risk factors increased with age;4. The combination of obesity, dislipidemia and hypertension was the most common combination of MS individual components;5. Visceral obesity was the most important risk factors of MS. UA, TC, history of hypertension and WBC were also risk factors. Objectives1. To detect the serum concentration of MMP-2 in MS patients and the correlations of MMP-2 and each component and risk factors of MS based on the result of epidemiologic study;2. To research the correlation of MMP-2 and methylation status of CpG sites in the MMP-2 promoter region according to the former result.Methods1. Screenning 75 MS patients and 82 heathy people from 2003 dwellers;2. Measuring the serum concentration of MMP-2 by ELISA;3. The methylation status of CpG sites in the MMP-2 promoter region was determined by MSP and BSP;4. Statistics: data was calculated by SPSS 13.0 package via methods of Spearman's correlation, chi-square-est and t-test.Results1. The serum concentration of MMP-2 in MS patients was significantly lower than that in control group.2. The concentration of MMP-2 was negatively correlated with TG, BMI, WC and DBP, but positively correlated with age.3. we didn't find methylation sites in the MMP-2 promoter region in this study both in MS patients and healthy control group.Conclusions1. The serum concentration of MMP-2 in MS patients was significantly lower than healthy control group and negatively correlated with TG, BMI, WC and DBP, the clinic significance and correlated mechanism of which needed further studied.2. we didn't find methylation sites in the MMP-2 promoter region in this study both in MS patients and healthy control group.