Effect Of Basic Fibroblast Growth Factor On Proliferation And Migration In Cultured Human Lens Epithelial Cells In Vitro

Objective:To investigate the effect of basic fibroblast growth factor(bFGF)on proliferation and migration in the cultured human lens epithelial cells(hLECs)and to explore the possible mechanism of bFGF in the formation and development of the posterior capsule opacification (PCO) ,which provide a new possibility for anti-PCO therapy.Methods: 1. The hLECs(SRA01/04)were cultured and sub-cultured. The sub-cultured cells from the third to fifth generation were used in this experiment following obveration of the shape. 2. The hLECs cultured in serum-freemedium were treated with bFGF in different concentration (0.01,0.10,1.00,10.0,100.00μg/ L))and following culturing in different time (24h,48h and 72h ), the MTT assay was used to detect the effect of proliferation. 3. To observe the cell growth cycle with Flow cytometry (FCM ): the hLECs were treated with 10μg/L of bFGF which induced the medium proliferation of the cells. After 24h and 48h, the changes of the growth cycle among the treated cells were detected with FCM. 4. An wound-healing model in vitro was used to detect the migration of hLECs following being treated with different concentration of bFGF (0.01,0.10,1.00,10.00,100.00μg/ L) for 24 hours.Result: 1. MTT assay:The bFGF in different concentration 0.1μg/L,1.00μg/ L,10.0μg/ L,100.00μg/ L all had significant effect on increasing the proliferation rate of cultured hLECs with density dependence compared with the negative control group ( P<0.05 or P<0.01). The hLECs treated with bFGF 100.00μg/ L for 24 hours showed the maximal increase of proliferation (P<0.01)。2. FCM analysis: the G0/G1-phase cells decreased while S + G2/M phase cells increased in the groups treated with bFGF 10μg/L for 24 hours and 48 hours. There was significant difference in the cell growth cycle between the bFGF groups and the negative control groups (P<0.05); This result confirmed that the bFGF can induce the proliferation by promoting the hLECs crossing the G1 cell cycle into the S + G2/M phase 3.The bFGF had significant effect on the migration of hLECs: the migration ability of hLECs increased by 27.21%, 154.42%, 238.77% at the concentration of bFGF 1μg/L,,10μg/L,100μg/L respectively with density dependent compared with the negative control group (P<0.01)Conclusion: bFGF can promote the proliferation and migration of hLECs in vitro and play a role in the development of PCO as a powerful mitogen and potent migratory factor for hLECs。...