Basic Fibroblast Growth Factor On Rabbit Lens Epithelial Cell Proliferation And Drug Interaction Experimental Research

Purpose Basic fibroblast growth factor (bFGF) is one of the cell factors which are widely distributed in the body. It can stimulate many kinds of cell to splitting and proliferating,can also stimulate lens epithelial cells, corneal epithelial cells, endothelial cells and retinal capillary endothelial cells proliferation.In recent years, opthalmologists increasingly pay attention to the effect of bFGIF on lens epithelial cells. The purpose of these experiments was to study the promotive effect of bFGF on the proliferation of rabbit lens epithelial cells(RLECs); and to study the mutual effect of basic fibroblast growth factor and Cisplatin (PDD), Mitoxantrone(Mit) and Cytosine Arabinoside(Ara-c) on rabbit lens epithelial cells. Methods Lens epithelia were isolated and cultured, and 2~棐3 passage RLECs were placed in 96-well flat bottomed tissue culture plates and incubated for 24 hours, lO~-?lOng/mi concentrations of bFGF were added,and the proliferation characteristics of the cells were measured by a rapid colorimetric assay (MTT assay). On the basis of HE staining ,the expression of proliferating cell nuclear antigen(PCNA) was observed with immunocytochemicai staining. Cell ultrastructure was observed by transmission electron microscope (TEM). The change of cell cycles was 4 observed by flow cytometer (FCM).The effect of different concentrations of Cisplatin, Mitoxantrone and Cytosine Arabinoside for 24 and 72 hours on RLECs was studied separately. Different concentrations of bFGF were then added, and the mutual effect of bFGF and these drugs was observed. Results After the treatment with bFGF ,RLECs showed marked proliferation; the expression of PCNA showed positive staining with bFGF;TEM showed cells became more active with bFGF, FCM showed that the S-phase cells increased, and that cell function became more active. And while PDD and Mit showed marked inhibition effects, Ara-c produced no obvious effects in this experimental condition. When bFGF was added, PDD,Mit and Ara-c also showed marked inhibition. Conclusion These experiments confirmed that bFGF has powerful proliferation effects on RLECs.The effect was enhanced as concentration and time increased.bFGF can抰 change the inhibition effects of the drugs(PDD and Mit); and can enhance the inhibition effect of Cytosine Arabinoside on RLECs.This work provides an experimental basis for the mechanism and prevention of posterior capsular opacity after cataract surgery.