| Background: Ganoderma lucidum is Chinese traditional medicinal plant playing an important role in traditional Chinese medicine. There are about more than 200 kinds of ganoderma lucidum in the world, and China has 100 of them. Ganoderma is one of most common kinds. Most of study on pharmacological effects of Ganoderma lucidum is based on ganoderma as the object. Modern pharmacology also shows that ganoderma lucidum has many bioactivity components having many effects including hepatoprotection, improving microcirculation, slowing down senescence, improving neurasthenia and so on. Different methods for preparing ganoderma lucidum decide the different composition in it and the pharmacological effects. So far there are several kinds of ganoderma preparation, such as ganoderma lucidum ultramicro powder, ganoderma lucidum powder, ganoderma lucidum spore powder, wall-broken spores of ganoderma lucidum and so on with various efficacies. However the advantage or short among them is unkown. So it is very meaningful to find a kind of efficient ganoderma preparation to explore its mechanism of action and treat many kinds of diseases.Objective: This thesis mainly discussed the immunoregulation effects of ganoderma powder, ganoderma spore powder, wall-broken spores of ganoderma by detecting their immunoregulation effects on normal mice and immune suppressive mice, which is used for explore their mechanism of immunoregulation and find out a kind of effective ganoderma for improving immune functions.Method:1,Separating peritoneal macrophage and splenocytes in normal mice and detecting three kinds of the effects on these cells functions of ganoderma preparation using phagocytosis assay and lymphocyte proliferation assay.2,To obtain immunosuppressive mice model, we inject cyclophosphamide 75mg/kg.bw in abdominal cavity. Then we treated these mice with intragastric three kinds of ganoderma preparation 1g/kg.bw for two weeks. Finally, we detected the body weight and spleen index; macrophage function with peritoneal macrophage phagocytosis of chicken red blood cells assay; T and B lymphocyte proliferation and transformation function with lymphocyte proliferation assay; killing activity of NK cells with cytotoxicity assay and YAC-1 cell as the targeting cells; the percentage of CD4+ and CD8+ T cell with flow cytometry.Result:1,The effects on immune functions of normal mice by ganoderma preparations. Compare with control group, three kinds of ganoderma preparation had no effects on phagocytosis of macrophage, but a little suppression on T and B lymphocytes. These results indicated that the coculture of immune cells and ganoderma can not improve the functions of immune cells. It maybe due to metabolism of preparation in vivo which makes them better play function. So we performed the in vivo study with immunosuppressive model.2,The effects on immune functions of immunosuppressive mice by ganoderma preparations. The body weight recovered to the normal level in three groups of ganoderma treating mice compared to the immunosuppressive mice. Spleen index and phagocytosis activity of macrophage didn't change. The killing activity of NK cell was increased especially with wall-broken spores of ganoderma. Ganoderma could improve proportion of CD4+ and CD8+ T cell in peripheral immune system, three kinds of the preparations could all improve lymphocyte function, especially wall-broken spores of ganoderma.Conclusion:1,Three kinds of ganoderma couldn't affect the macrophage function of normal mice, but had a certain suppressive function on T and B lymphocyte proliferation with dose-dependent, higher suppressive function in higher concentration. This is probable due to the incomplete effects in vitro or the high concentration.2,Three kinds of ganoderma preparation could recover the body weight of immunosuppressive mice, and improve the NK cell activity and T and B lymphocyte proliferation, especially the wall-broken spores of ganoderma. In addition, ganoderma powder could increase the proportions of CD4+ and CD8+T cells which play a role in regulating the innate and adaptive immune functions. |
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