Effets Of Chinese Materia Medica Gout Mixture And Its Optimal Mixture Contained Serum On Expression Of Tnf-Aand IL-1B Human Vascular Endothelial Cell Induced By Urate

Objective:To study the effets of chinese materia medica gout mixture and its optimal mixture contained serum on expression of TNF-a and IL-13 in Human Vascular Endothelial Cell induced by Urate.Methods:36 wista rats were randomly divided into three groups, gout mixture group,optimal mixture group and normal control group.each group were respectively administrated with ten times concentration of gout mixture, gout optimal mixture and normal saline for a week, times a day. after an hour to gastric perfusion in the last time,killed rats,separated serum and diluted into 80%,40%,10% concentrations. Human umbilical vein endothelial cells were trained routinely after cells fusion up to 80%, Cells were digested and divided into 4 groups:normal control group, model group, gout mixture groups, gout optimal mixture groups.each group have high, medium and low concentration subgroup. normal group cells were stimulated with 100μg/ml culture medium,the other groups cells were stimulated with 100μg/ml urate solution.At the same time,normal group and control group were intervented with 80%,40%,10% concentration normal culture medium,but 80%,40%,10% concentration gout mixture and optimal mixture contained serum was used to intervent matching cells groups.after 48 hours,extrating transparent upper liquid, TNF-a and IL-1βwas detected with enzyme-linked immuno sorbent assay and residual cells did MTT assay.Results:The level of TNF-a and IL-1βin each group was significantlly higher than normal group(P<0.05),Compared with model group, level of IL-Lβof high and medium concentration gout optimal was significantly lower (p<0.05).Conclusions: gout optimal mixture can reduce the inflammatory injure of human vascular endothehal cell induced by urate by down-regulating the level of IL-1β. Objective:To study the effect of gout mixture and its optimal mixture on rats acute gouty arthritis induced by monosodium urate,and whether the optimal mixture showed more superior effect than gout mixture.Method:54 wista rats were randomly divided into nine groups.Control group, model group, western medicine group, gout mixture (high does,medium does,low dose) group,optimal mixture (high does, medium deos,low dose) group respectively were administrated of normal saline, indomethacin suspension, gout mixture of high, medium and low concentration, optimal mixture of high, medium and low concentration for 7 days,twice a day. rats acute gouty arthritis modles were estalished On the fourth day by monosodium urate solution (concentration of 25mg/ml,0.05 ml) injected into the ankle cavity, while 0.05 ml normal saline was injected into joint cavity of rats in control group. The body mass was detected before administration of drug,model estabilishment and day 1,2,3 after modle estabilishment. The perimeter (mean value) of right jiont was measured in the same position at desigenated time (Am and Pm in the same day before model estabilishment and 0 2,4,6,8,10,12,24,48,72 hours after model estabilishment). After 72 hours of model estabilishment,blood samples were collected from inner canthus to count leukocyte and detect level of serum interleukin-1β;Executing rats after collecting blood, ankles were fixed to do Histopathologic examination with 10% formalin.Results:Totally 54 rats were involved in the result analysis.The body weights increased ongoingly before administation of drugs.After modle estabilishment,the increase of body mass in high dose of gout mixture and optimal mixture group and wester medicine group were significantly higher than other groups;Before model estabilishment,the perimete was no significant difference between AM and PM in one day.After model estabilishment,the ankles of rats swelled,especially in model group.After drug treatment,the swelling level was decreased.The high concentration of gout mixture and optimal mixture group were signifianctly difference from model groups,but compare to wester medicine group,there is no difference; Numeration of leukocyte and level of IL-1βin model group,gout mixture and optimal mixture group were higher than that of control model,especially model group.Gout mixture and optimization mixture of high dose group were lower than model group;The Histopathologic results showed that gout mixture and optimal mixture might reduce gouty joint edema, improve inflammatory cells infiltrating and synovial hyperplasia, the higher concentration of gout mixture and optimize mixture,the better of therapeutic effect. But from above indexes, gout mixture and optimize mixture group had no difference.Conclusions:Gouty mixture and optimal mixture can effectively inhibit the acute gout arthritis of rats,and the curative effect is dose dependent,but the effect is no difference between gout mixture and optimal mixture.