| The liver disease have become the ninth-largest killer of the human. How to treat liver d-iseases is an important research topic for the medical profession. To seek a safe and effectivetherapy of hepatitis is the hot spot with great concern and research in the medical professionat home and abroad. Ancient medicine and modern research results show that the treatment ofdiseases of the Chinese medicine having the characteristics of the multi-target, cure andaftercare binding and less side effects, which do not have in western medicine, are acceptedby more and more Chinese and foreign medical profession and patients. Rubus reflexus Kerbelong to Rubus, it is a kind of Chinese medicine which can antibacterial and anti-inflammat-ory, remove blood stasis, cure the rheumatism and strong your body. Local folk of Fujianwidely use it to treat various types of hepatitis. The basis of folk medicine is pretty fair.In this study, we use the Rubus reflexus Ker of Fujian Shanghang as the sample. thechemical composition of it were isolated by water extraction and organic solvent extraction.as a result, we get six components in all. They are water extraction, alcohol extraction,alcohol and petroleum extraction, alcohol and chloroform extraction, alcohol and ethylacetate extraction, alcohol and butanol extraction. Physical and chemical identification showthat these components contain flavonoids, saponins, polysaccharides, alkaloids andterpenoids.Mouse ear edema model and CCl4liver injury model was constructed to screen the sixcomponents in three dose gradient. The date processing used the Duncan's Multiple-Rangetest. The outcome reveal that the most efficacy composition is alcohol and ethyl acetateextraction,and its does is3mg/kg.2-DE separated and screened the liver protein of mouse injured by CCl4. Finally, we get51protein spot of differentially expressed, which contains39kinds of proteins identified bymass spectrometry(Tab.4.9). These proteins can be broadly divided into five groups:①CCl4injure liver of mice acute inflammation-related protein,②Protect liver and anti-oxida-tion-related protein,③signal transduction-associated protein,④material and energy-rela-ted protein,⑤molecular chaperones. Using bioinformatics to identify the above proteinfunction,suggesting that alcohol and ethyl acetate extraction efficacy mechanism may be:ind-ucing sulfite oxidase, serum albumin upregulated and stimulating glutathione synthesisand toenhance the SOD activity, eliminate oxygen free radicals, keep the liver cell membrane awayfrom oxidized; regulate the synthesis of lysine, folic acid, ornithine and S-adenosyl-L-methi- onine, to improve the body's immune system, remove the necrosis of liver cells whilestimulating the proliferation of liver cells, accelerated liver tissue repair, nourish and protectthe liver, returned ALT to normal levels, in addition,to the upregulated of acyl-CoA syntheta-se reduceing fatty acids reduce the damage to liver cells. The findings of Phospholipase andcalmodulin suggest that the phosphatidylinositol signaling pathway is involved in anti-infla-mmatory hepatoprotective effect. It is worth mentioning is that glucose-regulated protein78,mitochondrial heat shock protein60have been shown to inhibit cell apoptosisupregulated,prolyl4-hydroxylase plays an important role leading to liver fibrosis Cutexpress,these differentially expressed of molecular chaperones indicate alcohol and ethylacetate extraction component may have the effect of anti-cirrhosis. |
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