Research And Development Of A Bio-insecticide Gel Against Blattella Germanica

Germany cockroach (Blattella germanica) is a kind of very important sanitaryinsect pest in the world.They have been living for a very long time on the earth.Theycan live in the tropical zones,subtropical zones,temperate zones and frigid zones.There are many kinds of germs,virus and parasites in their mouths,especially in theirintestinals.Laboratory examination were done by scientists and the results showed thatthere were more than40kinds pathogens,like salmonella, dysentery bacillus,vitisbacillus,tubercle bacillus,pseudomonas aeruginosa,asporgillus, and more than7kindsof parasite ova and hepatitis virus,like roundworm and hookworm in cockroach’sinternal and extraneous body. Due to their unbeneficial living habits and potentialdamage to people,people have used many kinds of ways to kill them,includingchemical control,physical control and biological control,and so on.People abuse allkinds of cockroach control agents to kill them.These not only cost to much but alsoresult in the agent resistance of cockroachs. In order to improve controleffect,postpone the resistance of cockroach control agents and reduce the control costof cockroachs,this study conducted research and development on a kind of highlyactive,low toxic biological agents for cockroach control by ways of screening,laboratory toxicity experiments and laboratory efficacy simulation test.In this study，I made a preliminary screening to rotenone, matrine, teasaponinand boric acid for potential materials of cockroach insecticides by testing existingactive ingredients and attractants. The laboratory toxicity experiments showed that theefficacy of rotenone was not ideal, and matrine, teasaponin and boric acid can beconsidered as the further candidate materials.However,the price of matrine is tooexpensive.Finally,I chose to take the mixture of teasaponin and boric acid as theinsecticide material.Formulation tests showed that the CTC of three proportions ofteasaponin(A) and boric acid(B)(A:B=1:20，A:B=1:99，A:B=1:9) are141,146and151,far more than120,was obviously efficient.Mathematical model of the theoreticalsimulation for the mixture is:y=-0.19x~2+25.708x-712.935,the optimum ratio ofteasaponin and boric acid is3:17,and CTC is152.Laboratory toxicity experimentsshowed that CTC of practical optimum ratio was128.In order to test and validate the efficacy of above mixture,referring to the methodof GB/T13917.7-2009on insecticide testing standard,I have conducted manylaboratory toxicity simulation experiments.The results showed that3%consistency ofmixture gel bait of teasaponin and boric acid for controlling cockroaches was grade Aof national standard under the experimental conditions.It is worthy of further studyand for making marketization,which is expected to yield economic,social andecological benefit to people.