Study The Technology Of Extracting Sodium Alginate Via Mixed Microorganisms:Develop Mutagenic Breeding And Optimise The Conditions Of Fermentation

Sodium alginate is a natural polysaccharide and have a variety of industrial value, existing in seaweed. China has a strong production of kelp, but always waste a lot for the limited extraction yield in industry. Now, people are tend to use traditional method or enzymatic hydrolysis to extract sodium alginate, but traditional method has a low extraction yield, and although enzymatic hydrolysis can increase sodium alginate yield, but also increased the cost of production. So this topic choose cellulose enzyme, single bacteria and the mixed bacteria to processing kelp respectively, then choose the best method; processing Bacillus subtilis protoplast by He-Ne laster and screening the mutant strains with high cellulase enzyme activity and stable genetic; optimize the conditions of fermentation that extracts sodium alginate, for getting more efficient extraction technology, and make a possible that produce sodium alginate efficiently and cheaply.Processing kelp respectively by cellulose enzyme, single bacteria and the mixed bacteria, and compare the quality of sodium alginate, we can make sure that, when B181strains mixed with Bacillus subtilis, it will have the best effect, and increased the quality of sodium alginate12.86%than enzymatic hydrolysis.By this test we can know that under the conditions that lytic enzyme concentration is1mg/mL, enzyme solution time is60min, enzyme solution temperature is37℃, protoplast will have a higher yield and regeneration rate.Processing Bacillus subtilis protoplast by He-Ne laster at632.8nm wavelength, when the time reaches four minutes, the protoplast will the highest mutation rate, then choose the mutations strains by first screening and second screening, and determine the activity of produced cellulose enzyme, we can know that, the choosen regeneration strains produce a higher activity cellulose enzyme and a well hereditary stability of mutations. This topic by changing the conditions to foster the mixed bacteria, finding the best method for mixed bacteria is, when mixed B181strains and processed Bacillus subtilis BS-6with the ratio of1:2, and under the condition that30℃225rpm/min, then oscillate training36h can get the biggest extraction of sodium alginate.