| Capillary electrophoresis is a separation and analytical technique with the advantagesof high efficiency, low sample consumption, easy automation and simple equipment,which has raised more and more applications in biology, pharmaceutical, disease diagnoseand environments. Micellar electrokinetic chromatography is one separation mode toseparate neutral or ionic analytes with introduction of surfactant. In recent years, MEKChas increasingly recognized as a high effective separation technique for the quality controlof drug and plant components. In this experiment, micellar electrokinetic chromatographywas applied to analyze the content of the main bioactivity component-diosgenin in Di’aoXinxuekang capsules; at the same time, this method was applied to separate and quantifythe components of repellency effect in three essential oils.1) The application of micellar electrokinetic chromatography in quantitative analysis ofdiosgenin in Di’ao Xinxuekang capsulesDi’ao Xinxuekang capsule is made from the extract of the Chinese endemicmedicinal plant Dioscorea panthaica rhizome. It has significant clinical efficacy intreatment of cardiovascular disease. The quality of Di’ao Xinxuekang capsule wasnormally controlled based on the total content of steroidal saponins. However, literaturesshow that the hydrolyzed product-diosgenin is the absorbed metabolite and playing thepharmaceutical role in vivo. At present, there is no established technique for the qualitycontrol of Di’ao Xinxuekang capsule based on the quantitative analysis of bioactivitycomponent-disogenin.This paper for the first time develops micellar electrokinetic chromatography toanalyze the diosgenin content in Di’ao Xinxuekang capsules.The optimized conditions: an uncoated slica capillary column （50.2cm×75μm）,20mM borate buffer (pH9.5) and80mM (SDS) at a constant voltage of20kV andtemperature of25℃. The sample was injected at0.5psi for10s and theUV detectionwavelength was at195nm. The results showed the linearity was good over the range of10-100μg/mL for diosgenin (r=0.9954). The precision for migration time and peak height of diosgenin was lower than1.37%and4.53%, respectively. The recovery of diosgeninwas between99.17-102.14%. The content of diosgenin in Di’ao Xinxuekang capsule wasmeasured as12.5mg for each capsule.2) The application of micellar electrokinetic chromatography in separation andquantitative analysis of components of repellency effect in three essential oils.Clove bud oil, litsea cubeba oil and citronella oil are the essential oils extracted fromplants of clove bud, the root, stem or fruit of litsea cubeba and citronella, respectively.These three essential oils have significant effect of repellency to insects and mosquitoes.Eugenol, citral, linalool, limonene, citronellal and α-pinene are components of strongeffect of insects and mosquitoes repellency.In this experiment, micellar electrokinetic chromatography was first utilized toseparate and determine the contents of eugenol, citral, linalool, citronellal, limonene andα-pinene and successfully applied to analyze the components of repellency effect in threeessential oils.The optimized conditions: an uncoated slica capillary column （50.2cm×75μm）,20mM borate buffer (pH9.5) and50mM SDS at a constant voltage of30kV and temperatureof25℃. The sample was injected at0.5psi for5s and theUV detection wavelength wasat210nm. The results showed each component was in good linearity in certain ranges ofconcentration (r>0.996), the precisions for migration time ratio and peak area ratio weregood. The content of eugenol in clove bud oil was determined as89.60%; the contents of(Z)-citral,(E)-citral, linalool, limonene and a-pinene in litsea cubeba oil were26.48%,32.48%,4.08%,11.60%and1.76%, respectively; the contents of citronellal and limonenewere37.00%and4.14%, respectively. |
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