Study On The Liquid Fermentation Of Ganoderma Lucidum And Antioxidant Activity Of The Polysaccharide

As an important active ingredient present in G.lucidum, G.lucidum polysaccharide showsa lot of pharmacological activities, such as antioxidant activity, ant-aging, anti-tumor,immunomodulatory and blood-activating activities. In this dissertation, G.lucidum andG.sinensis were cultivated in liquid fermentation medium and the polysaccharides wereisolated and purified from the fermented mycelia. The antioxidant activity of fermentationbroth and polysaccharide factions were researched comparatively. The main results are listedas follows:1The liquid submerged fermentation conditions of G.lucidum and G.sinensis were researchedwith the biomass and exopolysaccharide as research objectiveThe results indicated that vinasse, glucose, wheat bran and yeast extract couldremarkably enhance the growth of mycelium and production of exopolysaccharide.According to the orthogonal experimental design, the optimal liquid fermentive medium ofboth species was showed as following(g/L): vinasse80, wheat bran7.5, been cake powder10,glucose5. G.lucidum grew faster and the growing trend was better than G.sinensis, but theoutput of G.lucidum exo-polysaccharide was lower than G.sinensis. G.lucidum and G.sinensisgrew in the pellets form. G.lucidum formed bigger, loose and fuzzy pellets, G.sinensis formedsmooth and hollow pellets.2The extraction technology of intracellular and extracellular polysaccharides were optimizedin this paperThe extracellular polysaccharide was precipitated by ethanol, the ethanol concentration,extraction temperature and solution pH were investigated. The results showed that theexopolysaccharide yield was highest(2.85g/L) when ethanol concentration was65%,extraction temperature was4℃and solution pH was5.The intracellular polysaccharide was extracted by hot water extraction and enzymaticextraction. The optimal extracting conditions of hot water extraction were showed asfollowing: extraction temperature95℃, extraction time3h, solid-liquid ratio1:3. In thisoptimum conditions the extraction yield of intracellular polysaccharide was1.78%. Theoptimal extracting conditions of enzymatic extraction were as follows: dosage of proteinase 1.5%, pH4.6, extraction temperature50℃, extraction time60min, then extracted at95℃for2h, the extraction yield was2.35%.3Isolation, purification and initial research of water-insoluble polysaccharide of G.lucidumand G.sinensisDEAE-cellulose52chromatography and Sephadex G-100gel column chromatographywere used for the isolation and purification of the cure polysaccharides of G.lucidum andG.sinensis. The results showed that, GLP were separated into two fractions byDEAE-cellulose52and Sephadex G-100, the two fractions were GLP-1and GLP-3respectively; GSP were also separated into two fractions, there were GSP-1and GSP-2. Thefunctional groups of the polysaccharides of G.lucidum and G.sinensis were studied throughUV Spectra, Infrared Spectra and HPGPC.4Antioxidant in vitro of fermentation broth and polysaccharides of G.lucidum and G.sinensisThe antioxidant activities of fermentation broth, cure polysaccharides and purepolysaccharides of G.lucidum and G.sinensis were studied in vitro, the results showed that,fermentation broth, deproteinized fermentation broth, cure polysaccharide and purepolysaccharide all had remarkable antioxidant activity; The antioxidant activities offermentation broth, cure polysaccharide and pure polysaccharide were compared, and theantioxidant activities of cure polysaccharide were compared with Vc. The results provide thefoundation for the research and utilization of liquid fermentation of Ganoderma.