The Therapy Effects Of Sorafenib On Human Lung Adenocarcinoma Cell Line A549/DDP In Vitro

Objective: To evaluate the effects of sorafenib on the physiological activity of humanlung adenocarcinoma cell line A549/DDP in vitro in order to provide Scientificexperimental data and theoretical basis for the treatment of cisplatin-resistant lungcancer.Methods: Human lung adenocarcinoma cell line A549/DDP was cultured in vitro.The group which only plus cell culture medium was served as control group(S0group).The groups which plus four concentration gradient of sorafenib (2、4、8、16μmol/L)were set to four experimental groups(s1~s4). The growth curve ofA549/DDP and A549cell line was described by MTT assay. The cell growthinhibition ratio with sorafenib for24、48、72hours was measured by MTT assay. Theapoptosis rate of control group and experimental groups for72hours was analyzed byflow cytometry. And the invasion effect was assessed by transwell chamber method.Results: MTT assay result displayed that Sorafenib could significantly inhibit theproliferation of A549/DDP cell within the concentration range from2μmol/L to16μmol/L. After treated with sorafenib for24hours, the inhibition ratio were4.58±2.816%、14.93±2.620%、37.58±7.132%、58.39±8.148%. Treated with sorafenibfor48hours the inhibition ratio were14.98±2.931%、26.28±7.308%、63.00±3.049%、78.84±3.956%.Treated for72hours the inhibition ratio were18.80±2.815%、32.71±2.547%、75.51±4.725%、87.50±3.361%.The inhibition ratio of A549/DDP cellincreased progressively with different time and dose(p<0.05).Flow cytometry resultshowed that A549/DDP cell appeared obvious apoptosis after treated with sorafenibfor72hours. The apoptosis of control group was8.88±0.81%.The apoptosis ofexperimental groups were2.84±0.24%、17.27±0.78%、21.98±0.75%、49.67±1.38%.The cell apoptosis rate of each experimental group was significantly higher than control group with concentration dependent raise. Transwell chamber method resultillustrated that the average A549/DDP cells passing trough the membrane were lessthan control group after treated with sorafenib for24hours. The control group cellnumber passing trough the membrane was (82.7±2.3). And the experimental groupswere (58.2±2.5)/HP、(41.3±1.3)/HP、(22.6±2.1)/HP、(14.7±1.1)/HP. In addition theshape of passing cells changed.Conclusions: Sorafenib could inhibit the proliferation of A549/DDP.And itsinhibition was time-and concentration-dependent manner. Sorafenib could alsoinduce the apoptosis and inhibit the invasion of A549/DDP.