| Articular surface is inevitable due to the complexity and continuity ofhuman motion, repeated exposure to friction and deformation of bodydysfunction, can cause varying degrees of wear of the articular cartilage, andbrought a series of pathological problems. There are3600to40million patientswith osteoarthritis of the millions of patients the need for joint replacement, atthe same time every year there are a large number of patients because of theaccident the need for joint replacement therapy. Scholars to the development ofvarious prosthesis based on the characteristics of the natural articular cartilage tosolve such problems, the study found that the prosthesis implanted in the bodyto the body under load, the installation of joint prosthesis in the knee itself complex movement while surface and with the surface generated by the frictionwear and tear, eventually lead to aseptic loosening of the prosthesis.In order tominimize the loosening of the prosthesis, pre-tested bio-tribological propertiesof the natural knee joint cartilage and the prosthesis is to reproduce clinical wearmechanisms, the performance of the prosthesis close to natural cartilage,thenatural articular cartilage tribology acts research Biotribology.Joint tribological is an important subject of the biomechanics. For mostpeople joints under a lot of the load, resulting in the case of high contactpressure to ensure smooth movement for70years, mainly due to the lubricatingproperties of the low coefficient of friction of the articular cartilage. Althoughscholars have conducted years of research, but on the Joint tribologicalmechanism of long-term effective lubrication process is still not very clear,natural articular cartilage in vitro Joint tribological test for joint health,diseaserehabilitation, medical implants, bionic mechanical significance.A large number of studies using different cell sources, scaffolds and growthfactors to stimulate and accelerate the regeneration of cartilage tissue, but only aminority of the actual conversion of the feasibility of clinical treatmentprograms, even in the articular cartilage of these methods long-termperformance of the functions is unclear. More tissue-engineered cartilage invitro study assessed the focus on cell biology and biochemical characteristics ofthe new materials to analyze and the similarity of the natural articularcartilage.However, only a few studies have investigated the natural cartilagebiomechanics and friction and wear properties,in fact, the latter has moresignificance. Need further study, the tribological characteristics of the naturalarticular cartilage tissue engineering materials in the cell inoculation density, incubation time, the structure and function, friction and other aspects moresimilar to natural cartilage.Although many in vitro studies have as cartilagesubstitutes (for example),tribological properties of the hydrogel material toassess, but few scholars can make a reasonable test of articular cartilage and atthe same time clarify the friction of articular cartilage. The main reason is thatthe shorter the time to maintain the natural cartilage away from the body afterthe biological characteristics of the determination of the values is limited to thedynamic analysis on a longer period of natural articular cartilage tribology.Maintain the time to find a convenient and effective cartilage preservationmethods to extend the activity of cartilage biology is one of the solutions.The most common way to preserve the articular cartilage at home andabroad the following, but have their own inadequacies:1,fresh frozen methodhas the advantage of simple, convenient, the disadvantage is that:â‘ the fewersurviving cells;â‘¡Strong immunogenicity;2,Cryopreservation method has theadvantage will enable organizations to save for a period of extendedshortcomings may damage the cartilage cells of the donor;3,yophilized orfreeze-drying Preservation Act of advantages for the preservation of moreconvenient,the disadvantages as those:â‘ leading to degeneration of the entirematrix;â‘¡The organization of enzymes occurred destruction lead to degenerationof the entire matrix;â‘¢prone to physical shrinkage;4,the advantages of the lowconcentration to better maintain the biological characteristics, the disadvantageis that easy to cause shrink.In this study, to explore the preservation of the natural cartilage, raisedunder high pressure, homemade FM preservation solution can longer maintainthe biological activity of the chondrocytes.6-8weeks time to meet the requirements of the articular cartilage in vitro tribology test. The experiment ona regular basis to take part of the articular cartilage histology, chemistry, cellviability, ultrastructural, immunohistochemical detection.Experimental resultsshow that under pressure pressurized FM preservation solution group in save1,3,6,9,cell morphology and cell ultrastructure of articular cartilage in generalnormal matrix mucopolysaccharide content rich, high cell viability;underpressure UW preservation solution group, under normal conditions,UWpreservation solution group and gradient cooling group, the preserved cells aremostly the cell membrane rupture, cell pyknosis, reduce the mucopolysaccharidecontent of the cell matrix, organelles The structure is unclear, after statisticalprocessing that the low cell viability. Antigen expression rates lower after thepressurized FM preservation solution group to save the articular cartilage,underpressure UW preservation solution group, there were significant differencesbetween the UW preservation solution group under normal conditions,withstatistical significance (P<0.05). Different preservation methods on the OD ofeach group (absorbance value) between the influential and groups havesignificant differences in the activity of: pressurized FM preservation solutiongroup is conducive to maintaining articular chondrocytes, articular cartilagedamage is smaller. The articular cartilage cell viability and to reduce the rate ofantigen expression, pressurized FM save the liquid group pressure UWpreservation solution group, under normal conditions, the UW preservationsolution group between statistical significance (P<0.05); the pressure FMpreservation solution group and gradient cooling group cryopreservation was nosignificant difference for the maintenance of articular cartilage, fibrocartilagecell activity and reducing the rate of antigen expression, but more convenient, the protective effect stronger. By MTT assay measuring the OD is worth toknow the activity of chondrocytes,the pressurized FM preservation solutiongroup in the3and6weeks and the pressure UW preservation solution group,under normal conditions,the UW preservation solution group compared withstatistical difference (P<0.05);9weeks There were significant differencesbetween the gradient cooling group (P <0.05).Experiment two selectedspecimens of the meniscal tissue,grouped with FM pressurized preservationsolution group, pressure UW preservation solution group,the gradient coolinggroup and the non-pressurized UW fluid group,mainly observed by opticalmicroscopy and electron microscopy,each group of specimens microscopicchanges. |
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