Dynamic Change Of Glutamate And GABA In The Treatment Of Rat Model Of Parkinson’s Disease

ObjectiveL-dopa treatment of PD is the golden criterion. By observing the changes ofglutamate, GABA and behavior of L-dopa treated PD rats, and exploring the dynamicrelationship between glutamate and GABA to provide the basis for follow-up L-dopatreatment.MethodsAccording to the factorial design,12successful modeled male SD rats weredivided into two groups (n=6): L-dopa group and vehicle group. L-dopa group andvehicle group on the1st,3rd,5th,7th,14th day were injected L-dopa and saline byintraperitoneal injection respectively, and used high performance liquidchromatography (HPLC) to detect the amount of glutamate, GABA in the dialysateweekly, lasted for four consecutive weeks, while observing the rotation behaviorchange by apomorphine.Results1. Three weeks after the rat model were made, the concentrations of glutamate andGABA in the striatal dialysate of PD group was2.41μg/ml and0.93μg/mlrespectively; and the concentrations of glutamate and GABA in the vehicle groupwas2.36μg/ml and0.81μg/ml.2. Four weeks after the rat model were made, L-dopa group glutamate concentrations lower than that of vehicle group about5times in dialysate. With the treated timeand the decline of rate of recurrence, the level of glutamate showed a gradualupward trend.3. Four weeks after the rat model were made, the concentrations of GABA higher thanvehicle group about15%. Along with the extension of therapy time and the declineof therapy rate, the level of GABA decreased gradually.4. On the behavior of L-dopa group, after two hours’ therapy, the rotation start timesignificantly getting shorter, then extended gradually in five days; With the extensionof treatment time, the number of rotations increased in two experimental groups, butthe number of rotations did not change significantly before and after treatment.ConclusionsIntraperitoneal injection of L-dopa can reduce the concentration of glutamate in thestriatum of PD rat, increase the GABA concentrations, and L-dopa improve the rotationstart time by apomorphine.