The Mechanism And Effect Of C-peptide On The Proliferation And Migration Of Umbilical Vascular Smooth Muscle Cells Induced By Insulin

Objective: To investigate the possible mechanism and effect of C-peptide on theproliferation and migration of umbilical vascular smooth muscle cells induced byinsulin.Methods:(methyl thiazolyl tetrazolium,MTT)MTT test was applied to determine theinhibition ratio and the relationship between the duration MTT chromatometry testwas applied to determine the proliferation of VSMCs; Boyden chambers were used toobserve the migration of VSMCs；By using Western blot,the phosphorylation statusof Akt and ERK was detected.Results: Insulin augmented VSMCs proliferation and migration;effects wereabrogated by C-peptide. C-peptide affect insulin-induced Akt or extracellularsignal-regulated kinase signaling.Conclusion: Our data support a causative role for insulin in VSMCs with a novelcounter-regulatory effect of proinsulin C-peptide.Thus,C-peptide can limit thedetrimental effects of insulin on VSMCs function.Co-supplementing insulin therapywith C-peptide could improve therapy in insulin-treated patients. C-peptide ablatesthe effects of insulin on VSMCs maybe acting by inhibiting the ERK or PI3K/Akrpathways, the two major pathways that lie downstream of the insulin receptor.