Researches On Several Key Techniques Of Quality Contral Of Rhizoma Corydalis

Rhizoma Corydalis, the dry rhizome of Corydalis yanhusuo (Fumariaceae), is one of the famous traditional herb medicine named as "Zhe ba wei". It is widely used in China for promoting blood circulation, reinforcing vital energy and alleviating pain. The alkaloids are considered to be the main bioactive components. In the present research, several key techniques of quality control in production links were studied, so that scientific suggestions can be proprosed for quality assessment and quality control of Rhizoma Corydalis, as well as its sustainable development. The main results are listed as following:1. Applying HPLC-DAD technique and multivariate statistic analysis, both chemical fingerprints and four alkaloid components quantitative analysis were combined to construct the chemical diversity evaluation system of Rhizoma Corydalis. This evaluation system provides technical means for the present study and quality evaluation of Rhizoma Corydalis.2. Through analysis and comparison of the chemical diversity of Rhizoma Corydalis from different regions and different geo-sources in a common garden experiment, we found that environment played a more important role in chemical diversity of cultivated populations than genetic background which may be due to low genetic diversity and Nantong environment is more conductive to its accumulation based on the content of THP which is the index component of Rhizoma Corydalis. But which specific factor in environment playing a major role should further study. And from a long-term sustainable use point of view, we should collect wild germplasm resources with high genetic diversity which can be applied to agricultural practice for breeding, so as to increase the active ingredient contents of C. yanhusuo and their ability of adaption to the environment.3. Study on the boiled mechanism of C. yanhusuo confirmed that boiling have a significant impact on the quality of Rhizoma Corydalis and revealed a process of changes of alkaloid contents during boiling. The mechanism during boiling of C. yanhusuo relates to that the leaching of alkaloids into boiling water mainly and thermal degradation of some alkaloids. The results support the processing method of Rhizoma Corydalis stipulated in Pharmacopeia of China, and further suggest that the processing method is boiling to the point when the white color of the central part of the rhizome disappears rapidly with water above 80℃.4. Having finished study about the effect of different drying methods and grades on chemical diversity, we suggest that air drying is prior to other drying methods and found that alkaloids of bulbs mainly distribute in the cortex, so the smaller the bulbs, the higher the content of index components. The results do not support the current requirement that the higher the specifications, the higher quality level.