The Protective Role Of Adenovirus Vector Mediated Interleukin10(rIL-10) Gene On Type1Diabetes Mellitus Of NOD Mice

Objective To investigate the protective role of adenovirus vector mediated interleukin10(rIL-10) gene on type1diabetes mellitus of NOD mice.Methods Culture293cell to amplify adenovirus. Forty-six NOD mice aged from4to5weeks were randomly divided into four groups. Group1is normal control group with10mice without special disposal. Groups2to4which contains12mice in each of them received in traperitoneal injection of Cyclophosphamide200mg/kg· d on the1st and14th day in order to induce type1diabetes mellitus. Random blood glycemia were measured after injection of Cyclophosphamide, higher than16.7mmol/L is regarded as early diabetes mellitus. Group3received intraperitoneal injection of adenovirus vector mediated rIL-10gene0.1ml. Group4received intraperitoneal injection of adenovirus vector mediated eGFP. Three weeks later, all mice were executed and fixed pancresta in formalin for12-24hours, then embedded in paraffin, and sectioned. We observed the incidence of diabetic mellitus,the degree of insulitis and the expression of IL-10detected by immunohistochemistry.Detected IL-10, IL-4, INF-γ, C-peptide by enzyme linked immunosorbent assay.Results Compared with group1, average blood glucose level and body weight increase are different from group2,3and4, but there is no difference among group2,3and4, so does inflammatory infiltration. Immunohistochemistry show that rIL-10has a higher expression in group3than group1,2and4. IFN-γ, IL-4, IL-10and C peptide have no difference in group2,3and4detected by ELISA.Conclusion The protection of adenovirus vector mediated IL-10is not obvious on type1diabetes mellitus of NOD mice.