Effects Of IGF-â…¡ Gene Silencing On Proliferation And Apoptosis Of Human Adrenocortical Tumor Cell Line SW-13

Objective To investigate the effect of IGF-II gene silencing by shRNA on growth and apoptosis in human adrenocortical tumor cell line SW-13and the PI3K/AKT signal pathway.Methods ShRNA lentiviral vector that can express shRNA of IGF-II was constructed, and transfected into human adrenocortical tumor cell line SW-13. SW-13cells were divided into three groups:blank control group, negative group and experimental group. After transfection, the mRNA was detected by Reverse transcription-polymerase chain reaction.Cell proliferation and apoptosis were determined with MTT and flow cytometry.Results ShRNA expression vector of the construction of the building by sequencing confirmed success. Compared with IGF-II-scrambled group and Blank control group, the expression of IGF-II, PI3K, AKT mRNA (0.95±0.006) E-04,(0.13±0.004) E-04,(0.64±0.108) E-04,was decreased significantly in IGF-Ⅱ-shRNA group(P<0.01); cell proliferation was significantly inhibited in IGF-Ⅱ-shRNA group (P<0.01); cell apoptosis was significantly higher in IGF-Ⅱ-shRNA group than in blank control group and negative group (P<0.01)Conclusion The shRNA of IGF-Ⅱ gene can effectively inhibit SW-13cell proliferation and promote cell apoptosis. And through the lower Akt, PI3K mRNA expression level, significant inhibitory PI3K/Akt signaling pathways. IGF-Ⅱ might be a potential target for the therapy of adrenal cortical carcinoma.