Expression Of Autophagy-related Genes Beclin1in Sp Cells And NSP Cells Of Human Lung Adenocarcinoma Cell Line A549

BackroundLung cancer is one of the most common malignant tumors in the world.In many countries,lung cancer is on the top of the list about death causing by cancers. People’s health is threatened by lung cancer, many patients are suffering it and lots of money is spent on it.Many hospitals and docors have paid much attention on this disease, so many new methods for treatment and diagnosis are under research.Although the step of progress for treatment in lung cancer is really slow, we still have some breakthrough in biochemistry,human genomics and cancer stem cell. For example:the screening of molecular markers about early diagnosis of lung cancer;found the suppressor genes TSLC1/CADM1in non-small cell lung cancer;use the difference between gene sequences and expression patterns,we can distinguish adenocarcinoma and squamous cell carcinoma;Through the genetic testing, predict the postoperative recurrence rate of lung cancer;the doctrine of cancer sten cells;separation and purification of tumor stem cells;SP cells and NSP cells were been separation successful;the progress in the research of cancer stem cell is improving, and so on. Although many research results are not used in clinic, they still have brilliant future in many ways. Some scientists have successfully separated SP cells from cancer cells, they also find that SP cells have traits of stem cells. Autophagy is becoming one of the most popular things in the cancer research. Some scientists have found differences in the expression of autophagy gene Beclinl and LC3between the tumor tissue and the normal tissue, they also believe the differences have some relationships with the stem cell traits. In this context,to explore the expression of Autophagy-related Genes Beclinl in SP cells and NSP cells of human lung adenocarcinoma cell line A549,around the difficult problem that the stem cells purification,though the SP cells, the purpose to the stem cells of lung cancer,stem cell autophagy is becoming one of the new methods for cancer treatment.ObjectiveTo explore the expression of Autophagy-related Genes Beclinl in SP cells and NSP cells of human lung adenocarcinoma cell line A549,to find the differences between SP and NSP cells,to provide some basis and foundation for the next drug experiments,about the adjustment of Autophagy-related Genes.Materials and MethodsHuman lung adrenocortical carcinoma cell line A549was cultured properly in vivo. SP and NSP cells in cell line A549were isolated by FACS respectively,the expression of autophagy-related genes Beclinl were detected by Western blot.ResultSP and NSP cells were isolated by FACS,the expression of Autophagy-related Genes Beclinl in SP cells was2.795±0.090, and in NSP cells was3.048±0.124. t=5.220, P<0.05, there were significant diference.ConclusionsThe expression of Autophagy-related Genes Beclinl in SP cells was lower than in NSP cells.There may have some relationships with the trait of tumor stem cell in SP cells,the adjustment of Autophagy-related Genes will become a new methods to treatment tumors.