| Background and Objective:In order to solve the shortage of donor kidneys, the use of elderly living donor kidney were increasing in recent years. However,older donor kidney during the course of organ transplantation sustained and functional damage after ischaemia/reperfusion remains unknown.The purpose of this study was to assess elderly donor kidney on renal allograft long-term effect and safety evaluation of elderly donor.Research methods:①50clean healthy male Sprague-Dawley(SD) rats,30elderly(18～20months) rats,20young(3～4months) rats. The aged rats were randomly divided into two groups:single kidney group (Aged group n=15), ischemia/reperfusion group (Aged IR group n=15); Adult rats were also randomly divided into two groups: single kidney (Adult group n=10), Adult ischemia/reperfusion group (Adult IR group n=10).(2)We established aged and adult SD rats renal ischemia/reperfusion and single kidney model to examine blood urea nitrogen (BUN), serum creatinine (Scr), kidney tissue related indexes and to observate kidney pathological morphological changes..③To compare the difference of BUN,Scr and the kidney morphology change between Aged and Adult,Aged IR and Adult IR.④.The gene expression of cytokines such as TGF-β, IL-6and Caspase-3mRNA were detected by real-time RT-PCR at8wk after ischaemia/Reperfusion.Results:1. The blood urea nitrogen (BUN) of Aged IR was slightly lower than Adult IR group,but not obvious at4wk after ischemia/reperfusion (9.770±0.403VS8.149±1.953,P=0.1359); there is significant difference between the single kidney groups to examine BUN,the young was higher than the aged (9.411±0.804VS6.945±0.824,P<0.0001). Aged IR group was higher in the detection of serum creatinine (Scr) than Adult IR(99.78±5.010VS79.53±5.117, P<0.0001); however,there is no statistical difference in levels of Scr between two groups with single kidney at4wk (86.76±7.330VS85.33±2.172, P=0.5838)2.①The levels of serum BUN decreased in aged IR at8wk after IRI significantly compare to young rats (7.248±0.929VS8.630±0.830, P=0.0072),while serum creatinine (Scr) was higher than young IR group with significant difference (98.68±3.583VS72.06±7.462, P<0.0001).There is no statistical difference in levels of BUN and Scr between two groups with single kidney at8wk (8.975±1.185VS8.083±0.767,83.05±5.855VS80.03±3.178,P P=0.082>0.05).②Compare with the Adult IR, Aged IR group showed the high cytokines mRNA levels in tissue including IL-6and TGF-β at8wk ((0.057%±0.042%VS0.011%±0.006%,0.250%±0.127%VS0.129%±0.036%; P=0.0078and0.0231); There is no significant difference between two groups with single kidney in comparison of IL-6,but the levels of TGF-β mRNA was higher than Adult group (0.157%±0.073%VS0.089%±0.052%, P=0.0364). The gene expression of Caspase-3mRNA were higher in the aged rats than in young rats (0.095%±0.044%VS0.052%±0.013%,0.106%±0.052%VS0.015%±0.013%) P=0.0177and0.0002respectively.The difference has statistical significance.③Renal histopathology was observated small quantities of focal segmental glomerulosclerosis, tubular atrophy and interstitial fibrosis in aged rats; after ischemia-reperfusion injury is more significant, and accompanied by lymphocyte infiltration. The renal tissue of adult group was no obvious abnormalities in morphology.Conclusion:This study demonstrated that①Health-older rats had a long-term survival time after resection for donor kidney, and the kidney was provided with a certain compensatory function.②The impact of elderly kidney after ischemia/reperfusion was significantly greater than the young kidney. There was an increased amount of kidney injury and the inflammatory response change in aged rats when compared to young rats. |
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