Studys On The Alkaloids And The Impact Of Processing On The Chemical Composition In Cortex Magnoliae Officinalis

The stem bark of M. officinalis (cortex Magnoliae Officinalis) has been extensively used as a traditional Chinese medicine for treatments of abdominal distention and pain, dyspepsia, and phlegm of cough with asthma. The main chemical constituents in cortex magnolia officinalis are lignans like magnolol, volatile oil like β-eudesmol,alkaloids like magnocurarine and magnoflorine. phenylpropanoid glysocides like magnoloside A. etc. Fried with ginger juice is a commonly used processing method which is in order to reduce irritants on throat and also harmonize stomach. Many scholars have studied impacts of processing on the contents of magnolol and honokiol in cortex magnolia officinalis. However, there are fewer reports about its influence on alkaloids and phenylpropanoid glysocides.There are ten alkaloids components isolated from cortex magnoliae officinalis which includes three aporphine alkaloids. Asimilobine(4). Magnoflorine(5).4-Keto-magnoflorine (6) and four Benzylisoquinoline alkaloids,(-)-Magnocurarine(1),(+)-Oblongine(2),(+)-Tembetarine (3).3.4-Dehydromagnocurarine (7) along with three amino alkaloids, N-Feruloylputrescine(trans)(8). N-Feruloylputrescine(cis)(9). Betaine(10). Six of them are quaternary ammonium alkaloids. Among them (6) and (7) are new chemical compounds. The characteristic fragmentation behaviors of alkaloids including aporphine alkaloids, benzylisoquinoline alkaloids and amino alkaloids were investigated systematically by electrospray ionization tandem mass spectrometry in positive ion mode. The relationship between their structural features and the corresponding characteristic fragmentation behavior was also investigated. It was found that the formation of six-membered cyclic structure influenced the fragmentation behavior of the aporphine and benzylisoquinoline alkaloids. The various type of alkaloids could be identified by determination of their ESI-MS" spectra. The results providing useful information for rapid determination of aporphine and benzylisoquinoline alkaloids in crude alkaloids extracts.During this research, cortex magnoliae officinalis was firstly roasted with ginger juice. Then the high performance liquid chromatography(HPLC) method was used to determine the contents of lignans. phenylpropanoid glycosides. and alkaloids in the raw and processed products. The volatile flavor components were analyzed by gas chromatography/mass spectrum(GC-MS). We also investigated influence of processing temperature on the contents of chemical components in cortex magnoliae officinalis. The results are as follows.1. The contents of lignans. phenylpropanoid glycosides and alkaloids in processed products were similar with the crude ones and the content of phenylpropanoid glycosides decreased by50%. It was indicated that the processing could lead to a decrease of phenylpropanoid glycosides’content in cortex magnoliae officinalis. Compared with unprocessed samples, no big difference was observed on the chemical constituents, but we found the change of the contents of volatile oil. and aslo we found the main chemical constitution of processing material like a-curcumene and a-aingiberene at the high boiling point scope.2. The contents of magnolol and honokiol were stable at80～160℃. The contents of syringing, magnoloside B and magnoloside A decreased with the rise of the temperature at the range of120～160℃. Magnoflorine had a poor thermal stability at120℃for its content dropped by40%.