Lingnan Upper Respiratory Tract Infection Cases Observed And Reated To Immune And Inflammatory Mechanism

Objective:Our research bases on the previous clinical experience, integrates dehygrosis with syndrome differentiation and mediation to cure viral upper respiratory tract infection. By way of prospective clinical research, we make an observation about patients who get viral upper respiratory tract infection and syndrome differentiated as damp heat. We mensurated the serum levels of T-lymphocytes CD3+%、CD4+%、CD8+%、NK%, free radical SOD、MDA、NO, the viral antibody IgM and calculated CD4+/CD8+. Accordingly we may reveal the biochemical basis and mechanism of damp heat syndrome in viral upper respiratory tract infection. Wormwgod and Scutellaria Gallbladder Clearing Decoction can clear away heat and expel dampness, we use this decoction to cure the disease in order to prove theupper respiratory tract infection.Methods:We selected42patients as case group, which diagnosed as viral upper respiratory tract infection from the medicine clinic and emergency clinic in First Affiliated Hospital of Guangzhou University of Chinese Medicine, GuangZhou Hospital of Integrated Traditional and Western Medicine, GuangDong Hospital of Traditional Chinese Medicine, and20normal cases as control group. Case group and control group were native people lived in southern areas in China. According to syndrome differentiation, case group was devided into two groups, which were damp-heat syndrome group(27cases) and warm-heat syndrome group(15cases). Indirect immunofluorescence was used to detect the blood serum for IgM antibody, T-lymphocyte subset was tested by flow cytometry, MDA、NO and SOD were determined by using bathyran chromatometry, nitrate reductase method, xanthine oxidase inhibition method. Compared the change of immune function and the level of free radical in damp-heat syndrome warm-heat syndrome group and control group. Wormwood and Scutellaria Gallbladder Clearing Decoction was used to cure Damp-heat syndrome group for three days, then used the same method to retest the T-lymphocyte subset and the level of free radical, Observed how the pricinple of clearing away heat and expelling dampness worked on damp-heat syndrome.Results:Damp-heat test results of inflammatory markers before and after treatment, analyzed by t-test, P<0.05, control group and western medicine; statistically significant. Results suggest:Haoqin soupinhibition on respiratory viral infections in patients with serumimmune inflammation of TNF-alpha, Damp-heat, the role of IL-2release, significant difference compared with the control group. Hot and humid group after treatment by the the Artemisiabaicalensis clear bile mixture, SOD activity increased (P<0.05),MDA and NO levels (P<0.05), suggesting that Haoqin mixture inanti-oxidation, to improve the free radical damage than Western medicine control the group has a good effect.Warm card detection of inflammatory markers in patients before and after analysis by the t test, P<0.05, results suggest that:Ma Xing Shi Gan Tang inhibition on respiratory viral infections Damp-heat the serum of patients with immune inflammatory factors TNF-alpha, IL-2the role of release, compared with control groupstatistically significant. Antioxidant, to improve the free radicaldamage, analyzed by t-test, P<0.05, comparison group, the Practitioners increased SOD activity after treatment (P<0.05),MDA and NO levels (P<0.05), suggesting that the Chinesemedicine treatment group has a good effect in anti-oxidation, toimprove the free radical damage.Damp-heat before and after treatment by flow cytometry results, analyzed by t-test, P<0.05, control group and western medicinetreatment group results were significantly different. Warm cardbefore and after treatment by flow cytometry results, analyzed by t-test, P<0.05, control group and western medicine treatment groupresults were significantly different.Conclusion:1. The pathological mechanism of damp-heat syndrome in upper respiratory tract infection has a certain link with peroxide radical damage, immune dysfunction, and this abnormal mechanism may induce the imbalance between yin and yang and the lingering and refractory pathological changes in damp-heat syndrome.2. Damp-heat syndrome in upper respiratory tract infection may have a strong connection with some respiratory virus infections, syndrome performance may related to the pathogenic features of the virus.3. The essence of damp-heat syndrome in upper respiratory tract infection relates to free radical damage, immune disorder, the pricinple of clearing away heat and expelling dampness can regulate the body’s immune function, reduce the free radical damage.