Experimental Study Of HA-TGF-β1Delayed Release Effect On The Rabbits Ear Cartilage Autograft

ObjectiveTo study the influence of the multiplication of cartilage cells,the expression of collagen Ⅱ and GAG content on NS and HA.To study the influence of the multiplication ofcartilage cells,the expression of collagen Ⅱ and GAG content on TGF-β1.To study theinfluence of the multiplication of cartilage cells,the expression of collagen Ⅱ and GAGcontent on HA as a drug carrier to synthesise HA-TGF-β1.To study the sustained releaseeffect of HA on TGF-β1and to sustainedly express the highly biological effect on TGF-β1.MethodsIn this investigation,32domestic rabbits were randomly divided into4groups:2weeks group,4weeks group,8weeks group and12weeks group,with8rabbits in each group.Avoiding the parts of the internal and external ear vein and artery,the cartilage tissue ofincluding the perichondrium is taken,1cm x1cm4block.After routine disinfection,4subcutaneous chamber are separated from the rabbit back with a depilatory cream off the fourregion,2cm x2cm.Then four pieces of cartilage are Implanted respectively.Incision suturesInterruptedly with3-0silk.Then each habbit was injected NS2ml,HA(100ug/L)2ml,TGF-β1(10ng/ml)2ml and TGF-β1(10ng/ml)+HA(100ug/L)mixture of2ml in the corresponding back subcutaneous cavity.In2,4,8,12weeks,the multiplication,the degeneration and the edge cells viability of cartilage cells were observed with HE staining,the expression of collagen Ⅱwas determined by immunohistochemical detection,and glycosaminoglycan(GAG)content was measured by the alcian blue colorimetric method.ResultsUnder the conditions of this experiment,the multiplication of cartilage cells,the expr ession of collagen Ⅱand GAG content in HA-TGF-β1group was higher than other groups after Cartilage transplantation(P<0.05),and the difference was statistically significant.Conclusions1．Under the conditions of this experiment,TGF-β1promotes the multiplication ofcartilage cells and maintains their synthesis of specific matrix.2．In the HA-TGF-β1slow-released system,HA as the carrier of TGF-β1has certainslow-released effect to TGF-β1and can delay the degradation of TGF-β1,so that HAenhances the ability that TGF-β1induces chondrocyte multiplication.3．HA-TGF-β1slow-released system makes cartilage cells continuously express the effect of high biological activity in local of graft,enables the cartilage cells to secrete thespecific collagen type II.The content of GAG is increased by the cartilage cells under the influence of the HA-TGF-β1.So that the cartilage grafts survives more easy.