Preliminary Study Of The Sulfur Dioxide Derivatives On The Toxicity Of Rat Ovarian Function

Background With the continuous development of industrialization and urbanexpansion, air pollution has become increasingly serious and has become threatto human survival environment and global sustainability. Sulfur dioxide （SO₂） isone of the major atmospheric pollutants. SO₂ are produced by the combustion ofcoal, oil, natural gas and a variety of non-ferrous metal smelting, oil refining,paper and vulcanized rubber on industrial, etc. SO₂ are volatile to air and leadto serious pollution. SO₂ as a toxic gas can cause the damage of brain, lung, heart,liver and other organ, severe cases can affect its function and lead to alife-threatening; Sulfur dioxide can cause systemic toxicity, and in the body,sulfur dioxide is derivatized into derivatives - sodium sulfite and sodiumbisulfite with the molar ratio of 3:1, then exists in the body and then distributedto the whole body with the blood.Objective To research the reproductive toxicity of sulfur dioxide bodyderivatives on female Wister rats’ovarian and the impact on sex hormone.The research could improve our understanding about the toxicity of sulfur dioxide,and thus strictly control the concentration of sulfur dioxide in the environment.Methods Adult female unpregnant WISTER rats were chose for the research, andsimultaneously they were healthy, 60 days and with body weight about（200g20）g（ Shanxi Medical University animal experiment center）. They were givenstandard light and particles fed at room temperature for 20-25℃and relativehumidity for 45% -55%. Vaginal discharges were taken at 9:00 to 11:00 daily morning.Vaginal discharges were smeared, papanicolaou stained and observed by lightmicroscopy. 30 rats were entered the experiment based on their regular sexual cycle.30 rats were divided into three groups at random: control group, high dose groupand low dose group. In Control group, the rats were infected in enterocoelia with1ml of normal saline, a daily injection for 14 days. In low dose group, the ratswere infected in enterocoelia with 1ml of sulfur dioxide body derivatives, withthe concentration of 0.5 g/kg，a daily injection for 14 days. And in high dosegroup, the rats were infected in enterocoelia with 1ml of sulfur dioxide bodyderivatives, with the concentration of 1 g/kg，a daily injection for 14 days.After intervention, the blood was collected in estrum. Detected the changes of follicle-stimulating hormone（FSH）, estradiol（E2）, corpus luteumerythropoietin（LH） with radioimmunoassay. We observed the pathological changesof ovarian glands.Results（1）The rats in high dose group reduced the diets and the actions, andafter intraperitoneal injection the rats occurred the symptoms of poisoning, suchas tremors and muscle cramps；（2）Compared three groups, FSH were significantlydifferent（F=84.19，P﹤0.0001）; Compared with control group, the levels of serumFSH were increased significantly in high dose group and low dose group （P<0.05）;and the impact of FSH in high dose group were higher than low dose group （P<0.05）.Compared three groups, LH were also significantly different（F=57.92，P﹤0.0001）;the levels of serum LH were significantly higher in high dose group compared withlow dose group and control group （P<0.05）. Compared three groups, E2 weresignificantly different（F=153.83，P﹤0.0001）; Compared with control group, thelevels of serum E2 were decreased significantly in high dose group and low dosegroup; and the impact of E2 in high dose group were super than low dose group（P<0.05）.（3）Pathology showed that primordial follicles and primary follicleswere increased in ovarian tissues of high dose group, but secondary follicles andmature follicles were decreased, and atresic follicles were also increased.Conclusion Sulfur dioxide derivatives could lead to the dysfunction of Gonadalaxis and the changes of ovarian tissues of female rats.