| Objective:Malignant ovarian tumor is one of the three kind of commonmalignant tumors in female reproductive system. The death rate of the femalewho suffer this tumor is the highest in gynecological malignancy. It hasalready become the main tumor which could seriously threaten women’s lifeand health. Ovarian tumor could conceal in the onset of illness, and it isextremely easy to infiltrate and transfer with patients no self-conscioussymptom. That is the most important reason why70%patients are in theterminal stage of cancer when first visit, and almost60%patients’ tumordiameter is over10cm when they get surgery in the first time. Advancedcancer patients who get operation, chemotherapy and some other medicaltreatments only have20%-30%chance to survive for5years. However,according to the relevant literature report, after early-stage ovarian tumor getappropriate medical treatment, the survival rate of5years of the patient couldachieve90%. Therefore, to research the pathogenesis of the ovarian tumor andto find out specific diagnostic index of the inchoate cancer are two significantevents which should be solved eagerly in the research of gynecological tumor.The reduce of the Puaster effect which means aerobic oxidation hasinhibition effect to the glycolysis and Crabtree effect which means it haspowerful glycolysis effect in the condition of glucose fully provided with nomatter there has oxygen or not exist in tumor cells. Not only Pyruvate Kinase(PK) is a key enzyme in glycolysis approach, but also it has a decisive roleduring the synthetic process of nucleoside triphosphate. Tumor type M2Pyruvate Kinase (Tu M2-PK) which has been more researched in recent yearsis a new kind of tumor markers. It shows preferable application prospect inearly diagnosis of malignant tumor.Monocarboxylic acid plays an important role in cell metabolism. The main physiological function of monocarboxylic acid is to provide energy tothe body. The function of lactic acid which is as to be the main finalmetabolite of the glycolysis is extremely special. The accumulation of lacticacid in cells could affect the activity of the phosphofructokinase, and that willcause the inhibition of the glycolysis. In the meanwhile, lactic acid is also thefuel of some kinds of cells’ breath. Therefore, the transport body which isresponsible for transferring lactic acid and some other single spindle acidsplays a key role to keep cells’ physiological function. In addition, glycolysis isthe main way to provide energy to tumor cells by themselves. It is thus clearthat no matter for the regular cells or tumor sells, the transport body of themonocarboxylic acid has significant meaning. MCTs is a key role in thetransportation process of lactic acid in cells.14members which namedMCT1-14have been found in mammals. Not only they have complexfunctions, but also they are widely extensive. Except monocarboxylic acid,there has transportation of many other foreign substances, such as hormone,medicine and something ales. According to the important function of MCTfamily which keep stable environment in cells by transferring lactic acid, it ishighly possible to have high expression in tumor. MCT1is widely extensive.Based on the research of MCT1’s inspection, it provides test evidences to theapplication of the malignant ovarian tumors’ diagnosis.Through using immunohistochemistry (IHC) to examine the expressionsituation of TuM2-PK and MCT1in human ovarian tumor tissue, the purposeof this research is to find out the meaning of TuM2-PK and MCT1protein inthe occurrence and development in ovarian cancer, and it provides testevidences to the further research of the early ovarian cancer’ diagnosis.Method：IHC S-P method is adopted to test66samples of the expressionof TuM2-PK and MCT1in ovarian cancer tissue and20samples of theexpression of TuM2-PK and MCT1in ovarian benign tumor tissue, and toanalyze their correlation and the correlation between their expression and theclinical pathology parameters by using SPSS13.0statistics software.Result: 1The expressions of M2-PK and MCT1proteins in ovarian cancer tissue andovarian benign tumor tissue.TuM2-PK protein almost doesn’t have expression in ovarian benigntumor tissue. However, it could be found the highly positive expression ofTuM2-PK which appears as many brownish yellow particles in cytoplasm ofovarian cancer cells. MCT1protein almost doesn’t have expression in ovarianbenign tumor tissue. However, it could be found the highly positive expressionof MCT1which appears as many brownish yellow particles on the envelope ofovarian cancer cells。 To compare with the difference of the positiveexpression rate of TuM2-PK and MCT1protein in both malignant ovariantumor tissue and benign ovarian tumor tissue has statistical meaning (P<0.05).The positive expression rate of TuM2-PK and MCT1protein is higher inovarian cancer tissue than it in the ovarian benign tumor tissue.2The correlation between the expression of M2-PK and MCT1protein andthe clinical pathology parameters。The positive expression rate of TuM2-PK and MCT1protein of ovariancancer patients relates to the tumor histological grade and clinical stage(P<0.05). However, it doesn’t have any connection with patients’ age or thetype of tumor pathology (P>0.05).3The correlation of the expression of TuM2-PK and MCT1protein.In ovarian cancer tissue, most of the time, both TuM2-PK and MCT1protein have express at the same time, and they present significant positivecorrelation. The coefficient of correlation is0.577, P<0.01.Conclusion:1The positive expression rate of TuM2-PK and MCT1protein is higher inovarian malignant tumor tissue than it in the ovarian benign tumor tissue.When ovarian cancer occur, the activity of the key enzyme which couldenhance glyconeogenesis has reducing, however, the activity and theexpression of the key enzyme that could enhance glycolysis have increasing,which would cause ATP losing its adjustment effect to glycolusis, and then ithas benefit to the growth of the tumor tissue. A lot of lactic acid have been made during the process of glycolysis, the accumulation of lactic acid in cellscould affect the activity of the Phosphofructokinase, and that will cause theinhibition of the glycolysis. The high expression of monocarboxylicacidtransportater protein which is on the cytomembrane could enhance lactic acidto transfer out from the cells. That has benefit to the growth of tumormalignant tissue.2The positive expression rate of TuM2-PK and MCT1protein of ovariancancer tissue relates to the tumor histological grade and clinical stage (P<0.05).However, it doesn’t have any connection with patients’ age or the type oftumor pathology (P>0.05). it could further explains that following thedevelopment of the cancer, tumor has been growing bigger and bigger, cellshave been increasing, TuM2-PK and MCT1which are combined by cells havealso been increasing. And it causes the increase of the positive expression rateof TuM2-PK and MCT1.3The expression of both TuM2-PK and MCT1present significant positivecorrelationship. And both of them participate in the occurrence anddevelopment of ovarian malignant tumor. Therefore, TuM2-PK and MCT1may be the tumor markers to the diagnosis of ovarian cancer. And they alsohave certain reference value to the prognosis of ovarian malignant tumor. |
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