The Expression Of MCT1 And CD147 In Human Gliomas And The Influence Of CD147 Genetic Engineering Antibody On Glioma Cells Glycolysis Metabolism In Vitro

OBJECTIVE:The present study was to explore the expression level of CD147 and MCT1 in human glioma cell,especially the diffirence of expression level between hige and low grade gliomas by using immunohistochemical localization.CD147 genetic engineering antibody is used to influence the expression of MCT1 on u251 cells,so that the lactic acid metabolism will be disturbed and cell growth will be delayed. MATERIALS AND METHODS1.specimens: from September 1998 to September 2000,60 surgical specimens were obtained from patients with gliomas,including 34 males and 26 females,ranging in age from 23 to 77 years(mean 58.2).There were 28 low grade and 32 high grade gliomas,according to the WHO classification.All specimens were fixed with 4% paraform and embedded in paraffin,and were routine serial section with the thickness of 4μm.