Effects Of Medium Chain Fatty Acid On Glucose And Lipid Metabolism In Kkay Diabetic Mice

Objective: Regarded the caprylic acid（caprylic acid, C8）and capricacid（capric acid, C10）as representative in medium-chain fatty acid,to observe the effects and correlated mechanism of medium-chainfatty acid on glycometabolism， lipid metabolism and body weight(BW) in diabetic KKAy mice, and to investigate the possibility ofmedium-chain fatty acid may act as improving the glucolipidmetabolism in diabetic patients.Methods:36male diabetic KKAy mice (aged4weeks) were dividedinto4groups randomly and each group contained12mice, and werefed with high-fat diet added caprylic acid(1.5%, C8), high-fat dietadded capric acid (1.5%, C10), and high-fat diet added oleic acidrespectively for12weeks. Food consumption and body weight wereobserved in mice of each group, to identify the difference of serumtriglyceride (TG), cholesterol (TC), free fatty acid (FFA),lipoprotein lipase (LPL), fatty acid synthetase (FAS), acetyl Coacarboxylase (ACC), food efficiency and blood glucose. The datawere analyzed by SPSS13.0software, and statistical significancewas designated at P<0.05bilaterally.Results: After12weeks, in caprylic acid group and capric acidgroup, the body weight, body weight increase value, and fat masswere lower than the oleic acid group. Compared with oleate acidgroup, fasting blood glucose decreased obviously with significantdifference (P<0.05) in Capric acid group; however, in glucosetolerance test, blood glucose in capric acid group decreased significantly after treated with glucose for half an hour, but thatvalue in oleate acid group had no degrade; blood glucose was stillrather low in capric acid group (treated with glucose for one and twohours), which were very significant difference in comparison tooleate acid group. The level of TG, TC, LDL-C, FFA in caprylic acidgroup and capric acid group was markedly higher than that of theoleate acid group (P<0.05), and the activity of LPL, FAS, ACC incaprylic acid group and capric acid group were lower than oleateacid group.Conclusion: On the premise of the same energy, caprylic acid andcapric acid can decrease markedly the body weight, fat mass andfasting blood glucose of the diabetic mice. In addition, capric acidcan improve the glucose tolerance. Medium-chain fatty acid may actby decreasing the activity of fatty acid synthetase and increasing theactivity of fatty acid oxygenolysis enzyme to reduce the body-weightof diabetic mice.