Effects Of Gestational Diabetic Mice On Glucose And Lipid Metabolism In Adult Offspring Mice

Objective To investigate the effect of gestational diabetic mice on glucose metabolism and lipid metabolism in adult offspring mice and to provide reference for clinical diagnosis and treatment.Methods Female and male mice of SPF grade 4 weeks old were selected and fed in cages at a ratio of 1: 1.After feeding,the vaginal test and vaginal test were used to further determine whether the mice had become pregnant.Twenty pregnant female rats were selected and randomly divided into a control group and an experimental group,with 10 rats in each group.The control group was treated with the same dose of sodium citrate buffer,and the experimental group was injected with streptozotocin 120 mg / kg intraperitoneally to establish an animal model of diabetes.The mice of the two groups were routinely reared until the offspring mice were delivered.At the time of birth,the two groups of offspring mice were measured for body weight,fasting blood glucose was measured by a blood glucose meter,and plasma leptin levels were measured by an enzyme-linked immunosorbent assay.Glucose Tolerance Test(GTT))Experiment and insulin tolerance test(Insulin Tolerance Test,ITT)experiment;Enzyme-linked immunosorbent assay to determine the serum leptin level of adult offspring mice;the blood lipid(TC,TG,LDL-C and,HDL-C)level;real-time fluorescence quantitative PCR detection of peroxisome proliferator-activated receptor α(PPARα),(carnitine palmitoyltransferase 1α,CPT1α),(enoyl-Co A,hydratase / Gene expression of3-hydroxyacyl Co A dehydrogenase(Ehhadh),(long-chain acyl-Co A synthetase 3,ACSL3)was detected by immunohistochemical staining.All data were processed using SPSS18.0 software.(1)The birth weight and fasting blood glucose of the offspring in the observation group were higher than those in the control group(P<0.05).Plasma leptin level in the observation group was lower than that in the control group(P < 0.05).(2)the blood glucose of the offspring mice in the observation group was higher than that of the control group at 30 min,60min and 90 min GTT after delivery(P < 0.05).The blood glucose level in the observation group was higher than that in the observation group at 30 min,60min and 90min(P<0.05).(3)the lipid levels of the offspring mice in both groups were measured 20 weeks after delivery,and the results showed that:the lipid levels of the offspring mice in the observation group at 20 weeks after delivery were higher than those in the control group(P<0.04)and lower than those in the control group(P< 0.05).(4)ITT was measured at 20 weeks after delivery of the two groups of offspring mice.The results showed that the expression levels of30,60 and 90 min in ITT experiment in the observation group were higher than those in the control group(P < 0.05).ITT test level was lower than 0min(P<0.05)at30min,60 min and 90 min in the observation group and control group.(5)liver-related gene assay was completed in both groups,and the results showed that the m RNA levels of PPAR level,CPT1 level and Ehhadh were higher in the observation group than in the control group(P<0.05).ACSL3 m RNA level was lower than that of control group(P < 0.05).ResultsConclusions Persistent hyperglycemia in GDM mice will lead to disorders of glucose metabolism and lipid metabolism in progeny,which may be related to increased expression levels of PPARα,CPT1α,Ehhadh factors in liver and decreased ACSL3 factors.