Expression And Significance Of MIR-134in Patients With Intractable Epilepsy And A Rat Model Of Epileps

PURPOSE：The expression of microRNA-134(miR-134), CREB andp-CREB were researched in temporal lobe foci from patients withintractable epilepsy and in hippocampus of experimental animals.METHODS: Twelve temporal neocortex tissue from patients withintractable epilepsy(IE) and twelve histological normal temporal lobesfrom controls were selected. Forty Sprague-Dawley(SD) rats were dividedrandomly into eight groups, including seven groups with epilepsy inducedby lithium-pilocarpine administration and one control group withoutepilepsy. And then hippocampal tissues were taken from rats at6hours,24hours,72hours,1weeks,2weeks,1months,2months after seizures, andfrom controls. Expression of miR-134, CREB and p-CREB were assessedby immunohistochemistry, western blot and real-time quantitative PCR.Results: In human brain tissues, mir-134expression of patients withintractable epilepsy was significantly lower than the controls(P<0.05). Andmir-134expression of epileptic rats was also lower than normal rats,especially these epileptic rats at time points of72hours,1weeks,2weeks,2months after seizures(P<0.05). CREB and p-CREB were in nucleus of neurons, and significantly higher in patients with intractable epilepsy(P<0.05). In epileptic rats, unlike upregulation of p-CREB expression at alltime points after seizures, upregulation of CREB expression was mainlydiscovered at72hours,1weeks,2weeks,2months after seizures(P<0.05).CONCLUSION： Abnormal expression of mir-134, CREB andp-CREB is likely to involve in MFS, abnormal formation of excitabilityloop and repeated seizures.