| Objective:The effects of transplantation of human umbilical cord blood mononuclear cells (HUMNCs) combined with atorvastatin on the angiogenesis in rabbits after acute myocardial infarction were investigated.Methods:In the Central Laboratory of Xiangya Hospital and Central South University Central South University animal experimental department., the experiments have been completed from2011-5to2011-12.①Human umbilical cord blood mononuclear cells were isolated, cultured, marked.②The model of acute myocardial infarction was established by ligation of the left anterior coronary artery (LAD). The60Chinese rabbits of the model built successfully were divided into four groups randomly. The control group (n=15) were injected intravenously with normal saline in24h after operation and gavaged with saline2ml daily. The atorvastatin group (n=15) were injected intravenously with normal saline in24h after operation and treated with atorvastatin5mg (Kg-d) by intra-gastric infusion. The transplantation group (n=15) were intravenously administrated with human umbilical cord blood mononuclear progenitor cells (HUCBCs) labeled with GFP3×107/500uL in24h and gavaged with saline2ml daily after myocardial infarction models. The combination group(n=15) were transplanted with HUCBC labeled with GFP3×107/500uL in24h after myocardial infarction models and treated with atorvastatin5mg (Kg-d) by intra-gastric infusion.③At1,2,4weeks after the treatment, cardiac funtions were performed by echncardiography; the number of transplanted cells in the myocardium was found by GFP positive cells counted with fluorescence microscopy. The microvessle density (anti-Ⅷ factor monoclonal antibody) and the expression of vascular endothelial growth factor (VEGF) in the myocardium were determined by immunohistochemistry staining.Results:(1) Compared to control group and atorvastatin group at1,2,4weeks after the treatment, the left ventricular fractional shortening (LVFS) and left ventricular ejection fraction (LVEF) were significantly improved in the transplantation group and the combination group. However the cardiac function was improved more obviously in the combination group compared to the transplantation group.(2) The GFP positive cells were found in the peri-myocardial infarction area in the both transplantation and combined groups at1,2,4weeks post-transplantation, and while the number of the GFP positive cells in the combined group was found more than that in the transplantation group.(3) Compared with control group at1,2, 4weeks after the treatment, the micro-vessle density and the expression of VEGF were significantly increased in the boundary of infracted myocardium in the three atorvastatin, transplantation and combined groups, and increased mostly in the combined group.Conclusion:The combination of atorvastatin and transplantation of HUMNCs for acute myocardial infarction can promote the therapeutic effects cooperatively. The possible mechanism may be that the combination of atorvastatin with intravenous transplantation of HUMNCs can enhance vascular regeneration and improve survival rate of grafted cells in the myocardium. |
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