Study On Simultaneous Determination Of The Content For Multi-index Components And Serum Pharmacochemistry In The Active Ingredients Of Smilax China L.

Rhizoma smilax.popularly known in China,belong to the liliaceous family Smilax china L., with swelling and analgesia, the role of blood circulation, expelling wind and dampness,was collected in the "the Chinese Pharmacopoeia"in2005and2010version, with its raw material is prepared Jin Gang Teng capsule in Hubei furen Pharmaceutical Company’s leading products for the treatment of gynecological inflammatory diseases, effieacy significantly.To control the quality of the Jin Gang Teng capsule from the source,to ensure that the clinical efficacy,we use serum pharmacochemistry research methods, combined with HPLC fingerprint comparative analysis, study the parts of effective anti-inflammatory substances into the blood after the prototype components and their metabolites presence and profile-effect relationship, reverse to trace Smilax efficacy of active substances (group), and also establish Smilax medicine active ingredients of the indicators with the spectrumthe determination method for the determination, in order to evaluate the quality of the herbs of Smilax more fully.By HPLC wavelength switching simultaneous,to establish an RP-HPLC method of simultaneous determination of the content for astilbin,Engelitin, resveratrol,quercetin4kinds of index components for the first time in the aqueous extracts of Smilax china L. The developed method has high sensitivity, good repeatability, reliability, which is a comprehensive evaluation of the quality of Smilax medicine,helpful to further improve the Smilax herbs quality standards that the2010version of "Chinese Pharmacopoeia" collection contains in the determination of the method.By using research methods of serum pharmacochemistry, to serum after rats oral the Smilax anti-inflammatory of the effective parts as the research object, the establishment of analysis methods on Smilax composition into the blood.by comparing HPLC differences of Smilax aqueous extract,Smilax containing serum and blank serum, identified45kinds of constituents absorbed in blood, including25kinds of prototype composition, the remaining20may be the prototype components of metabolic product. Addition this study found that astilbin,engelitin,resveratrol,quercetin as the main composition into the blood, which may be a direct role for the Smilax anti-inflammatory activity inside the body, secondary development of innovative new varieties of traditional Chinese medicine and to further provide a scientific basis.