Analysis Of The Composition And Its Metabolism In Vivo Of The Anti-inflammatory Effective Parts Group Of Smilax China

Chinaroot Greenbrier alias Jingangteng,its medicinal parts dry roots of liliaceous plant of Smilax China L.,containing in Chinese Pharmacopoeia 2015 version of A,with blood stasis and dispelling wind and dampness,detoxification effect,for rheumatism,urine dripping,leucorrhea,furuncle carbuncle disease,TCM clinical for treatment of gynecological inflammation,block,cancer and other diseases.Modern pharmacological studies show that sarsaparilla extract has obvious anti-inflammatory and anti-tumor effect.The prophase of existing listed species "Jingangteng capsule for secondary development of and by pharmacological experiment to determine the Smilax China with anti-inflammatory action of the effective parts,and by macroporous adsorption resin separation and purification technology was prepared mainly contain flavonoids and stilbenes and steroidal saponins component of the anti-inflammatory part group.This paper using ultra performance liquid chromatography / high resolution time of flight mass spectrometry(UPLC/Q-TOF-MS)of the effective fractions of Smilax anti-inflammatory ingredients were analyzed,and to study its in pelvic inflammatory disease model rats serum and metabolic product.The results of the study will provide the experimental basis for the screening of the active components and the study of the pharmacokinetics.Will enter the blood component and its metabolites as the starting point of the study of the efficacy of traditional Chinese medicine,can be more accurate interpretation of the biological activity of traditional Chinese medicine.This paper mainly completed the following work:1.Using ultra high performance liquid chromatography(UPLC)method the fingerprints of 10 batches of Smilax China L.were studied,marking the 35 common peak of Smilax in 10 major components were investigated.The results showed that 10 components in 10 batches of medicinal materials were obviously different.According to the fingerprint chromatogram and the determination results,the 2 batches of mixing ratio,including in Smilax China gap origin of raw material source,as Smilax effective part preparation of raw materials to ensure follow-up serum and pharmacokinetic dynamics research.2.The laboratory the prophase optimization of Smilax China the antiinflam matory effective parts of group technology for extraction and purification,prepared by the 10 batches of different extracts;uplc-dad fingerprints of 10 batches of extracts,were obtained with fingerprint peaks,marking the 33 common peaks,confirming the 13 characteristic peaks,10 batches of effective part group similarity of chromatographic fingerprint were greater than 0.9,showed that the extraction process is stable.The establishment of effective parts of the fingerprint of the Smilax anti-inflammatory group in vitro,which laid the foundation for the serum and metabolism research.3.Based on UPLC/Q-TOF-MS technology and principal component analysis(PCA)fragmentation patterns,the chemical composition of effective fractions of anti inflammation were preliminary inference.The UP LC/HRTOF-MS 58 chemical constituents were detected.Of the 47 chem ical components identified by mass spectrum information inference,inclu ding 20 components by reference to confirm,for effective fractions of later research and identification of Smilax in pelvic inflammation in rats in vivo prototype and metabolites laid a good foundation.4.the rat model of chronic pelvic inflammatory disease was prepared by injection of Escherichia coli and mechanical damage.The effective part of Smilax group irrigation stomach pelvic inflammatory disease model rats and collected serum and urine and feces were collected for detection of UPLC/Q-TOF-MS,and compared with the in vitro fingerprint and chemical composition information.Blood of the Communist Party of China(CPC)to detect the chemical composition of 42,including 18 prototype components,24,metabolites;urine detection of the Communist Party of China out of 107 chemical composition,of which 34 prototype components,shift,metabolites 73,which most of the metabolites as glucuronic acid of combination and sulfated combination;feces CPC identification the chemical constituents of 67,including 39 prototype components and shift for metabolites 28,feces mainly unabsorbed prototype components and some combination.The research results provide a scientific basis for the interpretation and clinical application of Smilax pharmacodynamic material basis.