Hepatoprotective Effect Of Uyghur Drug Cichorium Glandulosum And Determination Of The Major Active

Objective:1. To study the mechanism and protective effect of Cichoriumglandulosum on liver injury induced by carbon tetrachloride (CCl4) in mice.2. The majoractive constituents in C.glandulosum Boiss.et Hout were isolated, extraction andquantified. Method:1. Kunming mice were randomly divided into normal control group,CCl4model group, positive control group, low, middle, high dose groups ofC.glandulosum Boiss.et Hout and C. intybus extract. Experimental mice continuous oraladministration28days, normal control group and model group animals were given thesame volume distilled water. On the29th day, the mice except normal control group weregiven10mL/kg0.2%CCl4solution on oral. The activities of serum ALT and AST, as wellas the pathological changes of the liver were determined in all groups.2. Raw material ofC.glandulosum Boiss.et Hout was exhaustively extracted with Methanol, then theextraction was separated by silica gel column chromatography with petroleum-ethylacetate(2:1~1:2).3. The major active constituents in C.glandulosum have been quantifiedby UPLC. Result:1. Compared with the model group, all dose groups of the extractscould reduce serum ALT activity significant (P＜0.05). All dose groups of the extractscould decrease serum AST activity extremely significantly (P＜0.01). Histopathologicalobservations showed that extracts of C.glandulosum could ameliorate the liver injury indifferent degree.2. Lactucin, lactucopicrin and β-sitosterol were isolated from Cichoriumglandulosum.3. The major active constituents have a certain degree of difference.Conclusion: Coumarin and sesquiterpenoids of Cichorium glandulosum have significanteffect to protece chemical liver injury.