Three Kinds Of Plants In Xinjiang On Dipeptidly Peptidase â…£ Inhibition Activity

Objective: To observe the effect of three Xinjiang plants （fenugreek, chickpeas andpomegranate rind） water extract on peptide peptidase IV （DPP Ⅳ） activity in vitro andin vivo experiments. Methods:1.Experiment is using DPPⅣinhibition model, the threeXinjiang a hypoglycemic effect of plant extracts were screened in vitro.2.Selected90male mice, injection of streptozotocin （STZ）180㎎·㎏^-1in traperitoneal, establishmentof a diabetic mouse model in vitro, the model mice were randomly divided into modelgroup, treatment group, including the sitagliptin group, fenugreek low dose group (3g·㎏^-1), calabash Ba middle dose group (6g·㎏^-1), fenugreek high dose group (12g·㎏^-1),chickpea low dose group (1g·㎏^-1), chickpea middle dose group (2g·㎏^-1), chickpeas inhigh dose group (4g·㎏^-1), determination of mouse portal blood serum GLP^-1content andDPPⅣ activity effect, and the determination of related biochemical parameter aftergiving4weeks. Results: Fenugreek extract water IC500.03mg·mL^-1, extraction frompomegranate peel IC500.19mg·mL^-1, chickpea extract IC500.09mg·mL^-1.Compared whitthe normal control group with model group mice, the model group of weight significantreduces, fasting plasma glucose, total cholesterol, triglyceride, low density lipoproteinincreases, high density lipoprotein, peptide C levels decrease, decline in insulin secretion,insulin resistance increased, GLP^-1 decreased（P<0.05,P<0.01）. Fenugreek and chickpeawas administered for4weeks, compared with model group, fasting plasma glucose,totalcholesterol, triglyceride, low density lipoprotein decrease, high density lipoproteinsincreased significantly, C peptide levels increased, increased insulin secretion, insulinresistance decreased, with the rate of DPP IV inhibition increased, the content of GLP^-1was increased（P<0.05,P<0.01）. Conclusions: Experiments decreased the blood glucoseand blood lipid in diabetic mice, the possible mechanisms of action were dipeptidlypeptidase IV inhibitory activity in Xinjiang sugar aqueous extracts of plants and so on.