The Clinical Significance And Variation Of CD4~+CD25~+Foxp3~+Regulatory T Cells And IL-10、TGF-β1in Peripheral Blood Of Rheumatic Heart Disease Patients

[Objective] To find out the change of CD4+CD25+Foxp3+Treg cells, IL-10and TGF-β1in peripheral blood of the rheumatic heart disease(RHD) patients, to discover the relationship between the IL-10and TGF-β1, to further reveal the mechanism of the RHD, and to supply a important theory evidence for clinical diagnosis.[Methods] Flow cytometry was used to analyze the proportion of CD4+CD25+Foxp3+T cells/CD4+T cells in peripheral blood mononuclear cells (PBMCs) from40patients with RHD and40healthy peoples. Both who come from the hospital, and using the leavings serum of the same sample to test the concentration of IL-10, TGF-β1by ELISA. Clinical data of RHD patients were collected, and were used to analyze the relationship between the proportion of Treg cells in PBMCs and IL-10, TGF-β1.[Results] The CD4+CD25+Foxp3+Treg cells and IL-10, TGF-β1of patients with RHD are obviously decreased compare with the normal control. And the changes of IL-10, TGF-β1may have a relationship of direct proportion with CD4+CD25+Foxp3+Treg cells in PBMCs of patients with RHD.[Conclusion] Through study the clinical significance and variation of CD4+CD25+Foxp3+regulatory T cells and IL-10、TGF-β1in peripheral blood of rheumatic heart disease patients, we can conclude as the follows:1. The CD4+CD25+Foxp3+Treg may take important effect in the immunological regulation of RHD.2. The changes of IL-10, TGF-β1probably results from the change of CD4+CD25+Foxp3+Treg cells in PBMCs of patients with RHD.